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논문 기본 정보

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학술저널
저자정보
Wang Ying (Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and The Affiliated Cancer Hospital) Lu Kaijuan (Qidong Hospital of TCM) Li Weibing (Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and The Affiliated Cancer Hospital) Wang Zhigang (Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and The Affiliated Cancer Hospital) Ding Jing (Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and The Affiliated Cancer Hospital) Zhu Zeyu (Huaian Hospital) Li Zhipeng (Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and The Affiliated Cancer Hospital)
저널정보
한국유전학회 Genes & Genomics Genes & Genomics Vol.43 No.11
발행연도
2021.11
수록면
1,307 - 1,316 (10page)
DOI
10.1007/s13258-021-01160-6

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Background Gastric cell carcinoma (GCC) is a common and high-incidence malignant gastrointestinal cancer that seriously threatens human life and safety. Evidences suggest that microRNAs (miRNAs) exhibit an essential role in regulating the occurrence and development of GCC, while the efects and possible mechanisms remain to be further explored. Objective This study was designed to explore whether miR-200c-3p exerted its functional role in the growth and metastasis of GCC, and investigate the possible mechanisms. Methods The expression levels of miR-200c-3p in GCC tissues and cell lines were detected by qRT-PCR analysis. The functional role of miR-200c-3p in the viability, proliferation, migration and invasion of GCC cells were evaluated by CCK-8, EdU, wound healing and Transwell assays. In addition, the candidate targets of miR-200c-3p was predicted and confrmed by dual-luciferase reporter assay. Moreover, the relationship between miR-200c-3p and target (Kruppel like factor 6, KLF6) was assessed by qRT-PCR and western blot assays. Besides, the expression levels of KLF6 in GCC cells were determined by qRT-PCR and western blot assays. Furthermore, the role of KLF6 in the viability, proliferation, migration and invasion of GCC cells mediated with miR-200c-3p mimics was evaluated by CCK-8, EdU, wound healing and Transwell assays. Results In the present study, a new tumor promoting function of miR-200c-3p was disclosed in GCC. We found that the expression of miR-200c-3p was obviously increased in clinic GCC tissues and cell lines. In addition, down-regulation of miR-200c-3p suppressed cell viability, proliferation, migration, and invasion in GCC cells. Moreover, KLF6 was verifed as a direct target of miR-200c-3p by binding its 3’-UTR. Additionally, KLF6 was remarkably decreased and was negatively associated with the miR-200c-3p expression in GCC cell lines. Furthermore, over-expression of KLF6 retarded the efects of miR-200c-3p on the growth and metastasis of GCC cell lines. Conclusions MiR-200c-3p potentially played a tumor-promoting role in the occurrence and development of GCC, which may be achieved by targeting KLF6.
#Gastric cell carcinoma #MicroRNA-200c-3p #Kruppel like factor 6 #Proliferation #Metastasis

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참고문헌 (41)

참고문헌 신청
Vikram Agarwal / 2015 / Predicting effective microRNA target sites in mammalian mRNAs / eLife 4 Crossref Tapan Behl / 2020 / Intercalating the Role of MicroRNAs in Cancer: As Enemy or Protector / Asian Pacific Journal of Cancer Prevention 21(3):593~598 Crossref Chang-Zheng Chen / 2005 / MicroRNAs as Oncogenes and Tumor Suppressors / New England Journal of Medicine 353(17):1768~1771 Crossref Wanqing Chen / 2016 / Cancer statistics in China, 2015 / CA: A Cancer Journal for Clinicians 66(2):115~132 Crossref Xiaohua Chen / 2019 / MicroRNA‑21 regulates the biological behavior of esophageal squamous cell carcinoma by targeting RASA1 / Oncology Reports 41:1627~1637 Crossref

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