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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 105 - 114 (10page)
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Background Uncovering molecular pathogenesis and mechanisms of small cell lung cancer (SCLC) will contribute to SCLC therapy. Multiple studies demonstrated that miR-451a acts as an anti-tumor miRNA in non-small cell lung cancer. However, the mechanism of miR-451a in SCLC was ambiguous. Objective We aimed to explore the function of miR-451a in SCLC and decipher the underlying mechanisms. Methods TargetScan and dual-luciferase reporter assays were used to analyze the target genes of miR-451a. Cell counting kit-8 and colony formation assays were performed to assess the roles of miR-451a on cell growth. Gene set enrichment analysis (GSEA) was utilized to enrich biological pathways. Western blot was used to measure protein expression. Results MiR-451a expression was reduced dramatically in SCLC tissues and cell lines (NCI-H1688 and NCI-H446). Helicase, Lymphoid Specific (HELLS) was proved to be a target gene of miR-451a. In addition, cell proliferation assays in SCLC cells transfected with miR-451a mimic and/or HELLS revealed that miR-451a inhibited cell proliferation via targeting HELLS. Moreover, the roles of miR-451a/HELLS in expression of key proteins in mTOR and apoptosis signaling pathways suggested that miR-451a inactivated mTOR and activated apoptosis signaling pathway via directly silencing HELLS. Conclusions Our study indicated that miR-451a hinders SCLC cell proliferation in vitro through regulating mTOR and apoptosis signaling pathways via silencing HELLS, suggesting that miR-451a could be a promising tumor suppressor in SCLC. And there is a potential for miR-451a to be a drug target and biomarker for SCLC.
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참고문헌 (44)
참고문헌 신청
Dong-qin Chen / 2018 / HDAC3-mediated silencing of miR-451 decreases chemosensitivity of patients with metastatic castration-resistant prostate cancer by targeting NEDD9 / Therapeutic Advances in Medical Oncology 10:175883591878313
Lise Lotte Christensen / 2014 / Functional Screening Identifies miRNAs Influencing Apoptosis and Proliferation in Colorectal Cancer / PLoS ONE 9(6):e96767
Changwei Dou / 2019 / Hypoxia-induced TUFT1 promotes the growth and metastasis of hepatocellular carcinoma by activating the Ca2+/PI3K/AKT pathway / Oncogene 38(8):1239~1255
Akiteru Goto / 2017 / The low expression of miR-451 predicts a worse prognosis in non-small cell lung cancer cases / PLOS ONE 12(7):e0181270
Jia-Yuan Huang / 2015 / MicroRNA-451: epithelial-mesenchymal transition inhibitor and prognostic biomarker of hepatocelluar carcinoma / Oncotarget 6(21):18613~18630
Lise Lotte Christensen / 2014 / Functional Screening Identifies miRNAs Influencing Apoptosis and Proliferation in Colorectal Cancer / PLoS ONE 9(6):e96767
Changwei Dou / 2019 / Hypoxia-induced TUFT1 promotes the growth and metastasis of hepatocellular carcinoma by activating the Ca2+/PI3K/AKT pathway / Oncogene 38(8):1239~1255
Akiteru Goto / 2017 / The low expression of miR-451 predicts a worse prognosis in non-small cell lung cancer cases / PLOS ONE 12(7):e0181270
Jia-Yuan Huang / 2015 / MicroRNA-451: epithelial-mesenchymal transition inhibitor and prognostic biomarker of hepatocelluar carcinoma / Oncotarget 6(21):18613~18630
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