miR-582-5p inhibits migration and chemo-resistant capabilities of colorectal cancer cells by targeting TNKS2

Xiao Weixing; Zhou Haijun; Chen Bingrong; Shen Bin; Zhou Jun

doi:10.1007/s13258-021-01141-9

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자료유형: 학술저널

저자정보: Xiao Weixing (Jiaxing Hospital of Traditional Chinese Medicine China) Zhou Haijun (Jiaxing Hospital of Traditional Chinese Medicine China) Chen Bingrong (Jiaxing Hospital of Traditional Chinese Medicine China) Shen Bin (Jiaxing Hospital of Traditional Chinese Medicine China) Zhou Jun (Jiaxing Hospital of Traditional Chinese Medicine China)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.44 No.6

발행연도: 2022.6

수록면: 747 - 756 (10page)

DOI: 10.1007/s13258-021-01141-9

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Background Metastasis and chemo-resistance are still important factors that limit the overall efficacy of colorectal cancer treatment. Understanding the detailed molecular mechanism and identifying potential biomarkers are of great value in prognosis prediction and risk stratification. Objective We investigated the role of miR-582-5p in colorectal cancer pathogenesis, progression and chemo-resistance. Furthermore, we explored the underlying molecular mechanism of miR-582-5p in modulation of malignant behaviors of colorectal cancer cells. Methods Clinical samples and colorectal cancer cell lines were applied to explore miR-582-5p expression level and its significance on tumor cell metastasis and chemo-resistance. Transwell study and cellular survivability study were performed to explore the influences of miR-582-5p expression modulation on tumor cell chemo-resistance and invasion/migration. Dual-luciferase reporter gene assay was conducted to explore the influences of miR-582-5p on its target gene TNKS2. Results Colorectal cancer patients with lymph node or distal organ metastatic diseases exhibited significantly lower level of miR-582-5p. In vitro studies have indicated that miR-582-5p inhibition significantly increased migration and chemo-resistant capabilities of tumor cells. And dual-luciferase reporter gene assay demonstrated that miR-582-5p exhibited its influences on the biological behavior of tumor cells by targeting TNKS2. Conclusions Our study demonstrated for the first time that miR-582-5p played an important role for colorectal tumor cell metastasis and chemo-resistance. Our research also indicated that miR-582-5p and its target gene TNKS2 could be novel biomarkers for metastatic disease prediction, overall prognosis evaluation, as well as potential therapeutic target for colorectal cancer patients.

#miR-582-5p · TNKS2 · Chemo-resistance · Colorectal cancer · Biomarkers

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