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논문 기본 정보

자료유형
학술저널
저자정보
Bao Yuyan (Pharmaceutical Preparation Section Sanmen People's Hospital 15 Taihe Road Hairun Street Sanmen Coun) Yu Yanjie (Sanmen Market Supervisory Authority 5 Qiushui Avenue Haiyou Town Sanmen County Zhejiang Province Ch) Hong Bing (Pharmaceutical Preparation Section Sanmen People's Hospital 15 Taihe Road Hairun Street Sanmen Coun) Lin Zhenjian (Pharmaceutical Preparation Section Sanmen People's Hospital 15 Taihe Road Hairun Street Sanmen Coun) Qi Guoli (Pharmaceutical Preparation Section Sanmen People's Hospital 15 Taihe Road Hairun Street Sanmen Coun) Zhou Jie (Pharmaceutical Preparation Section Sanmen People's Hospital 15 Taihe Road Hairun Street Sanmen Coun) Liu Kaiping (Pharmaceutical Preparation Section Sanmen People's Hospital 15 Taihe Road Hairun Street Sanmen Coun) Zhang Xiaomin (Pharmaceutical Preparation Section Sanmen People's Hospital 15 Taihe Road Hairun Street Sanmen Coun)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제11호
발행연도
2021.11
수록면
1,508 - 1,518 (11page)
DOI
10.4014/jmb.2106.06031

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Hsa_circ_0001947 is associated with multiple cancers, but its function in non-small cell lung cancer (NSCLC) is ambiguous and needs further research. The targeting relationship among circ_0001947, miR-661, and downstream of tyrosine kinase 7 (DOK7) was predicted by database and further verified by dual-luciferase reporter assay, while their expressions in cancer tissues and cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR). After transfection, cell biological behaviors and expressions of miRNAs, miR-661 and DOK7 were determined by cell function experiments and qRT-PCR, respectively. Circ_0001947 was low-expressed in NSCLC tissues and cells. Circ_0001947 knockdown intensified cell viability and proliferation, induced cell cycle arrest at S phase, suppressed apoptosis and evidently enhanced miR-510, miR-587, miR-661 and miR-942 levels, while circ_0001947 overexpression did the opposite. MiR-661 was a target gene of circ_0001947 that participated in the regulation of circ_0001947 on cell biological behaviors. Furthermore, DOK7, the target gene of miR-661, partly participated in the regulation of miR-661 on cell viability. Hsa_circ_0001947 acts as a sponge of miR-661 to repress NSCLC development by elevating the expression of DOK7.

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