MicroRNA‑377‑3p inhibits growth and invasion through sponging JAG1 in ovarian cancer

Liulin Tang; Bin Yang; Xiaolan Cao; Qin Li; Li Jiang; Dan Wang

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자료유형: 학술저널

저자정보: Liulin Tang (Affiliated Hospital of Guilin Medical University) Bin Yang (Affiliated Hospital of Guilin Medical University) Xiaolan Cao (Affiliated Hospital of Guilin Medical University) Qin Li (Affiliated Hospital of Guilin Medical University) Li Jiang (Affiliated Hospital of Guilin Medical University) Dan Wang (Affiliated Hospital of Guilin Medical University)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.41 No.8

발행연도: 2019.1

수록면: 919 - 926 (8page)

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Background Ovarian cancer is the one of the most deadly gynecologic malignancy among cancer related death in women. However, the treatment for ovarian cancer is still limited. In this study, we aimed to explore the inhibition potential of miR- 377-3p in ovarian cancer and explore the mechanism of this effect. Methods Quantitative real-time PCR was used to detect the mRNA or microRNA (miRNA) levels. CCK-8, wound-healing, transwell assay were used to detect cell proliferation, migration and invasion. The protein levels were examined by western blot. The dual luciferase reporter assay was conducted to examine the luciferase activity. Tumor volume was measured and Ki67 was detected via immunohistochemistry. Results qRT-PCR results showed that miR-377-3p was downregulated in ovarian cancer patients. MiR-377-3p mimics suppressed cell proliferation, migration, invasion and decreased the JAG1 level. However, miR-377-3p inhibitor promoted these appearances. Interestingly, we found JAG1 was a target gene of miR-377-3p. JAG1 overexpression reversed the miR- 377-3p-induced inhibition of proliferation and invasion. In addition, miR-377-3p inhibited ovarian cancer tumorigenesis in vivo, indicating by decreased tumor volume and staining of Ki67. Conclusion The results showed that miR-377-3p inhibited growth and invasion of ovarian cancer cells by targeting JAG1.

#MiR-377-3p · Growth · Invasion · JAG1 · Ovarian cancer

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