Mesenchymal stem cell-derived exosome mir-342-3p inhibits metastasis and chemo-resistance of breast cancer through regulating ID4

Yu Shuyao; Zhou Yuhui; Niu Ligang; Qiao Yan; Yan Yu

doi:10.1007/s13258-021-01200-1

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자료유형: 학술저널

저자정보: Yu Shuyao (The First Affiliated Hospital of Xi’an Jiaotong University China) Zhou Yuhui (The First Affiliated Hospital of Xi’an Jiaotong University China) Niu Ligang (The First Affiliated Hospital of Xi’an Jiaotong University China) Qiao Yan (The First Affiliated Hospital of Xi’an Jiaotong University China) Yan Yu (The First Affiliated Hospital of Xi’an Jiaotong University China)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.44 No.5

발행연도: 2022.5

수록면: 539 - 550 (12page)

DOI: 10.1007/s13258-021-01200-1

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Background The mesenchymal stem cell-derived exosome (MSCs-exo) carrying microRNAs have been proved to regulate tumor biological activities. Clarifying molecular mechanism and identifying predictive microRNAs will be of great value in anti-tumor therapy improvement. Objective We aimed to investigate the regulatory role of microRNA-342-3p (miR-342-3p) in MSCs-exo on breast cancer. Methods Breast cancer tissues and cell lines were used to evaluate miR-342-3p expression in patients with or without lymph node/distal organ metastasis. The impact of MSCs-exo expression on tumor cell chemo-resistance and invasion/migration was measured. Dual-luciferase reporter gene assay was applied to identify binding site. Inhibitor of differentiation 4 (ID4) siRNA and miR-342-3p inhibitor transfection was conducted to further explore the miR-342-3p/ID4 axis on chemo-resistance and metastasis of breast cancer cells. Results Breast cancer cells revealed significantly lower level of miR-342-3p in patients with metastatic diseases. miR-342-3p suppressed invasive and chemo-resistant behavior of breast cancer tumor cells. Binding site between miR-342-3p and ID4 was proved. ID4 could reverse the influence of miR-342-3p on chemo-resistance. The tumor inhibition effect of IDA siRNA in vivo was also identified. Conclusions This study demonstrated that miR-342-3p acted as potential tumor suppressor by inhibiting metastasis and chemo-resistance of breast cancer cells through targeting ID4. This study might provide potential therapy targets for the treatment of breast cancer.

#miR-342-3p · Metastasis · Chemo-resistance · Breast cancer · ID4

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