지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.6
- 수록면
- 233 - 238 (6page)
이용수
초록· 키워드
오류제보하기
Pathogenic Naegleria fowleri, Acanthamoeba castellanii, and Acanthamoeba polyphaga, are distributed world¬wide. They are causative agents of primary amoebic meningoencephalitis or acanthamoebic keratitis in humans, respec¬tively. Trophozoites encyst in unfavorable environments, such as exhausted food supply and desiccation. Until recently, the method of N. fowleri encystation used solid non-nutrient agar medium supplemented with heat-inactivated Esche¬richia coli; however, for the amoebic encystment of Acanthamoeba spp., a defined, slightly modified liquid media is used. In this study, in order to generate pure N. fowleri cysts, a liquid encystment medium (buffer 1) modified from Page’s amoeba saline was applied for encystation of N. fowleri. N. fowleri cysts were well induced after 24 hr with the above de¬fined liquid encystment medium (buffer 1). This was confirmed by observation of a high expression of differential mRNA of nfa1 and actin genes in trophozoites. Thus, this liquid medium can replace the earlier non-nutrient agar medium for ob¬taining pure N. fowleri cysts. In addition, for cyst formation of Acanthamoeba spp., buffer 2 (adjusted to pH 9.0) was the more efficient medium. To summarize, these liquid encystment media may be useful for further studies which require axenic and pure amoebic cysts.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGMENT
REFERENCES
참고문헌 (20)
참고문헌 신청
[학술지(정기간행물)] - Ma P / 1990 / Naegleria and Acanthamoeba infections : review / Rev Infect Dis 12 : 490 ~ 513
[학술지(정기간행물)] - Schuster FL / 2004 / Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals / Int J Parasitol 34 : 1001 ~ 1027
[학술지(정기간행물)] - Zhang L / 1988 / Effects of cyclophosphamide and a metabolite, acrolein, on Naegleria fowleri in vitro and in vivo / Antimicrob Agents Chemother 32 : 962 ~ 965
[학술지(정기간행물)] - Visvesvara GS / 2007 / Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea / FEMS Immunol Med Microbiol 50 : 1 ~ 26
[학술지(정기간행물)] - Moon EK / 2009 / Autophagy protein 8mediating autophagosome in encysting Acanthamoeba / Mol Biochem Parasitol 168 : 43 ~ 48
[학술지(정기간행물)] - Schuster FL / 2004 / Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals / Int J Parasitol 34 : 1001 ~ 1027
[학술지(정기간행물)] - Zhang L / 1988 / Effects of cyclophosphamide and a metabolite, acrolein, on Naegleria fowleri in vitro and in vivo / Antimicrob Agents Chemother 32 : 962 ~ 965
[학술지(정기간행물)] - Visvesvara GS / 2007 / Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea / FEMS Immunol Med Microbiol 50 : 1 ~ 26
[학술지(정기간행물)] - Moon EK / 2009 / Autophagy protein 8mediating autophagosome in encysting Acanthamoeba / Mol Biochem Parasitol 168 : 43 ~ 48
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
비접촉 조건에서의 Naegleria fowleri에 의한 표적세포의 세포독성
한국가축위생학회지
2019 .01
-
Sirtinol Supresses Trophozoites Proliferation and Encystation of Acanthamoeba via Inhibition of Sirtuin Family Protein
Parasites, Hosts and Diseases
2022 .02
-
Naegleria fowleri Induces Jurkat T Cell Death via O-deGlcNAcylation
Parasites, Hosts and Diseases
2021 .10
-
DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation
Parasites, Hosts and Diseases
2017 .04
-
Acanthamoeba in Southeast Asia – Overview and Challenges
Parasites, Hosts and Diseases
2019 .08
-
Identification of Protein Arginine Methyltransferase 5 as a Regulator for Encystation of Acanthamoeba
Parasites, Hosts and Diseases
2016 .04
-
Antiamoebic activities of flavonoids against pathogenic free-living amoebae, Naegleria fowleri and Acanthamoeba species
Parasites, Hosts and Diseases
2023 .11
-
Can Acanthamoeba keratitis be properly diagnosed without culture in the real-world clinical microbiology laboratory?: a case report
Annals of Clinical Microbiology
2024 .06
-
Understanding the pathogenicity of Naegleria fowleri in association with N. fowleri antigen-1 (Nfa1)
Parasites, Hosts and Diseases
2024 .11
-
Identification and Characterization of Protein Arginine Methyltransferase 1 in Acanthamoeba castellanii
Parasites, Hosts and Diseases
2017 .04
-
Establishing a Cre/loxP-based genetic manipulation system for Acanthamoeba: Targeted genome editing and stable reporter expression
Parasites, Hosts and Diseases
2025 .02
-
Identification of essential genes for Acanthamoeba castellanii excystation during encystation and excystation
Parasites, Hosts and Diseases
2024 .11
-
Cytopathic Change and Inflammatory Response of Human Corneal Epithelial Cells Induced by Acanthamoeba castellanii Trophozoites and Cysts
Parasites, Hosts and Diseases
2019 .06
-
Interaction of Naegleria fowleri Trophozoites with Escherichia coli and MRSA by N-acetylglucosamine and Galactose
대한의생명과학회지
2021 .12
-
Characterization of a Peptide Antibody Specific to the Adenylyl Cyclase-Associated Protein of Acanthamoeba castellanii
Parasites, Hosts and Diseases
2022 .02
-
Isolation and Genotyping of Acanthamoeba spp. as Neglected Parasites in North of Iran
Parasites, Hosts and Diseases
2016 .08
-
Molecular and Biochemical Properties of a Cysteine Protease of Acanthamoeba castellanii
Parasites, Hosts and Diseases
2018 .10
-
Comparison of Proteins Secreted into Extracellular Space of Pathogenic and Non-pathogenic Acanthamoeba castellanii
Parasites, Hosts and Diseases
2018 .12
-
IL-1β and IL-6 activate inflammatory responses of astrocytes against Naegleria fowleri infection via the modulation of MAPKs and AP-1
대한체질인류학회 학술대회 연제 초록
2015 .05
-
Identification and Genotypic Characterization of Potentially Pathogenic Acanthamoeba Isolated from Tap Water in Wuxi, China
Parasites, Hosts and Diseases
2018 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2018-513-001176815