Cytopathic Change and Inflammatory Response of Human Corneal Epithelial Cells Induced by Acanthamoeba castellanii Trophozoites and Cysts

Hae-Jin Sohn; Ga-Eun Seo; Jae-Ho Lee; A-Jeong Ham; Young-Hwan Oh; Heekyoung Kang; Ho-Joon Shin

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자료유형: 학술저널

저자정보: Hae-Jin Sohn (Ajou University School of medicine) Ga-Eun Seo (Ajou University School of medicine) Jae-Ho Lee (Ajou University School of medicine) A-Jeong Ham (Ajou University School of medicine) Young-Hwan Oh (Ajou University School of medicine) Heekyoung Kang (Ajou University School of medicine) Ho-Joon Shin (Ajou University School of medicine)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.57 No.3

발행연도: 2019.6

수록면: 217 - 223 (7page)

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Acanthamoeba castellanii has ubiquitous distribution and causes primary acanthamoebic keratitis (AK). AK is a common disease in contact lens wearers and results in permanent visual impairment or blindness. In this study, we observed the cytopathic effect, in vitro cytotoxicity, and secretion pattern of cytokines in human corneal epithelial cells (HCECs) induced by A. castellanii trophozoites and/or cysts. Morphological observation revealed that panked dendritic HCECs co-cultured with amoeba cysts had changed into round shape and gradually died. Such changes were more severe in co-culture with cyst than those of co-cultivation with trophozoites. In vitro cytotoxicity assay revealed the highest cytotoxicity to HCECs in the co-culture system with amoeba cysts. A. castellanii induced the expression of IL-1α, IL-6, IL-8, and CXCL1 in HCECs. Secreted levels of IL-1α, IL-6, and IL-8 in HCECs co-cultured with both trophozoites and cysts were increased at an early incubation time (3 and 6 hr). These results suggested that cytopathic changes and pro-inflammatory cytokines release of HCECs in response to A. castellanii, especially amoebic cysts, are an important mechanism for AK development.

#Acanthamoeba castellanii #acanthamoebic keratitis #human corneal epithelial cell #cytotoxicity #pro-inflammatory cytokine

참고문헌 (29)

참고문헌 신청

Schuster FL / 2004 / Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals / Int J Parasitol 34:1001~1027

Marciano-Cabral F / 2003 / Acanthamoeba spp. as agents of disease in humans / Clin Microbiol Rev 16:273~307

Im K / 1998 / Acanthamoebiasis in Korea: two new cases with clinical cases review / Yonsei Med J 39:478~484

van Klink F / 1992 / Characterization and pathogenic potential of a soil isolate and an ocular isolate of Acanthamoeba castellanii in relation to Acanthamoeba keratitis / Curr Eye Res 11:1207~1220

Visvesvara GS / 1990 / Epidemiology of free-living ameba infections / J Protozool 37(suppl):25~33

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