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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.4
- 수록면
- 115 - 120 (6page)
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초록· 키워드
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Encystation mediating cyst specific cysteine proteinase (CSCP) of Acanthamoeba castellanii is expressed remarkably during encystation. However, the molecular mechanism involved in the regulation of CSCP gene expression remains unclear. In this study, we focused on epigenetic regulation of gene expression during encystation of Acanthamoeba. To evaluate methylation as a potential mechanism involved in the regulation of CSCP expression, we first investigated the correlation between promoter methylation status of CSCP gene and its expression. A 2,878 bp of promoter sequence of CSCP gene was amplified by PCR. Three CpG islands (island 1-3) were detected in this sequence using bioinformatics tools. Methylation of CpG island in trophozoites and cysts was measured by bisulfite sequence PCR. CSCP promoter methylation of CpG island 1 (1,633 bp) was found in 8.2% of trophozoites and 7.3% of cysts. Methylation of CpG island 2 (625 bp) was observed in 4.2% of trophozoites and 5.8% of cysts. Methylation of CpG island 3 (367 bp) in trophozoites and cysts was both 3.6%. These results suggest that DNA methylation system is present in CSCP gene expression of Acanthamoeba. In addition, the expression of encystation mediating CSCP is correlated with promoter CpG island 1 hypomethylation.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGMENT
REFERENCES
참고문헌 (19)
참고문헌 신청
[학술지(정기간행물)] - Aksozek A / 2002 / Resistance of Acanthamoeba castellanii cysts to physical, chemical, and radiological conditions / J Parasitol 88 : 621 ~ 623
[학술지(정기간행물)] - Turner NA / 2000 / Emergence of resistance to biocides during differentiation of Acanthamoeba castellanii / J Antimicrob Chemother 46 : 27 ~ 34
[학술지(정기간행물)] - 문은경 / 2011 / Microarray Analysis of Differentially Expressed Genes between Cysts and Trophozoites of Acanthamoeba castellanii / The Korean Journal of Parasitology 49 (4) : 341 ~ 347
[학술지(정기간행물)] - Moon EK / 2009 / Autophagy protein 8 mediating autophagosome in encysting Acanthamoeba / Mol Biochem Parasitol 168 : 43 ~ 48
[학술지(정기간행물)] - Song SM / 2012 / Autophagy protein 16-mediated autophagy is required for the encystation of Acanthamoeba castellanii / Mol Biochem Parasitol 183 : 158 ~ 165
[학술지(정기간행물)] - Turner NA / 2000 / Emergence of resistance to biocides during differentiation of Acanthamoeba castellanii / J Antimicrob Chemother 46 : 27 ~ 34
[학술지(정기간행물)] - 문은경 / 2011 / Microarray Analysis of Differentially Expressed Genes between Cysts and Trophozoites of Acanthamoeba castellanii / The Korean Journal of Parasitology 49 (4) : 341 ~ 347
[학술지(정기간행물)] - Moon EK / 2009 / Autophagy protein 8 mediating autophagosome in encysting Acanthamoeba / Mol Biochem Parasitol 168 : 43 ~ 48
[학술지(정기간행물)] - Song SM / 2012 / Autophagy protein 16-mediated autophagy is required for the encystation of Acanthamoeba castellanii / Mol Biochem Parasitol 183 : 158 ~ 165
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