지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.11
- 수록면
- 385 - 398 (14page)
이용수
초록· 키워드
오류제보하기
Naegleria fowleri, a brain-eating amoeba, thrives in lakes and rivers with aquatic vegetation and causes primary amoebic meningoencephalitis (PAM) in humans. Most recently, it has become such a serious problem that N. fowleri was detected in tap water in Houston, USA. Several pathogenic factors are considered very important to destroy target cells in the brain. In particular, the food-cup where N. fowleri antigen-1 (Nfa1) is located, is strongly expressed in pseudopodia involved in the movement of N. fowleri, and is involved in phagocytosis by attaching to target cells. In this article, we reviewed the role of the Nfa1 protein and its associated pathogenicity. The nfa1 gene was cloned by cDNA library immunoscreening using infection serum and immune serum. Nfa1 protein is mainly distributed in pseudopodia important to movement and vacuoles. Moreover, heat shock protein 70, cathepsin-like proteare and Nf-actin are also associated with pseudopodia in which Nfa1 is localized. Interestingly, the amount of the nfa1 gene changed as N. fowleri trophozoites transformed into cysts. Polyclonal antiserum against Nfa1 showed a protective effect against cytotoxicity of approximately 19.7%. Nfa1-specific IgA antibodies prevent N. fowleri trophozoites from adhering to the nasal mucosa, delaying invasion. The nfa1-vaccinated mice showed significantly higher levels of Nfa1-specific antibody. The duration of anti-Nfa1 IgG in the vaccinated mice lasted 12 weeks, strongly suggesting that nfa1 is a significant pathogenic gene and that Nfa1 is a pathogenic protein. Several factors related to pseudopodia and locomotion have been linked to Nfa1. A clearer function of N. fowleri targeting nfa1 with other genes might enable target-based inhibition of N. fowleri pathogenicity.
목차
Abstract
Introduction
Identification of nfa1 gene/Nfa1 protein using infection and immune sera
Analysis of basic components and functions of Nfa1 protein
RNAi showed that Nfa1 is a pathogenic factor
Effects of immunization with the recombinant Nfa1 (rNfa1) protein
DNA vaccine expressing nfa1 gene and application of lentiviral vector system
Several proteins including heat shock protein, cathepsin B, and other excretory proteins are involved in host cell invasion by pseudopodia
Nf-actin gene concerned with contact-dependent mechanisms in N. fowleri
Encystation of N. fowleri induced changes in nfa1 gene expression
Transcriptome profiling of N. fowleri revealed several genes involved in cellular mechanisms and immune responses
Conclusion
References
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
비접촉 조건에서의 Naegleria fowleri에 의한 표적세포의 세포독성
한국가축위생학회지
2019 .01
-
Naegleria fowleri Induces Jurkat T Cell Death via O-deGlcNAcylation
Parasites, Hosts and Diseases
2021 .10
-
Efficient Liquid Media for Encystation of Pathogenic Free-Living Amoebae
Parasites, Hosts and Diseases
2017 .06
-
IL-1β and IL-6 activate inflammatory responses of astrocytes against Naegleria fowleri infection via the modulation of MAPKs and AP-1
대한체질인류학회 학술대회 연제 초록
2015 .05
-
Interaction of Naegleria fowleri Trophozoites with Escherichia coli and MRSA by N-acetylglucosamine and Galactose
대한의생명과학회지
2021 .12
-
Metabolic Subtyping of Adrenal Tumors: Prospective Multi-Center Cohort Study in Korea
Endocrinology and Metabolism
2021 .10
-
Antiamoebic activities of flavonoids against pathogenic free-living amoebae, Naegleria fowleri and Acanthamoeba species
Parasites, Hosts and Diseases
2023 .11
-
Primary Amebic Meningoencephalitis: A Case Report
Parasites, Hosts and Diseases
2019 .06
-
Recent Progress in the Medical Therapy of Pituitary Tumors
Endocrinology and Metabolism
2017 .01
-
Quantitative changes innatural antibodies stimulated from a D-galactose-induced aging mouse model
Journal of Biomedical and Translational Research
2020 .01
-
Monitoring of Noxious Protozoa for Management of Natural Water Resources
Parasites, Hosts and Diseases
2018 .04
-
Pathogenicity of H5N8 virus in chickens from Korea in 2014
Journal of Veterinary Science
2015 .06
-
Pathogenic Analysis of Mouse-Adapted 2009 Influenza A (H1N1) Mutant Virus
한국실험동물학회 학술발표대회 논문집
2015 .08
-
Pathogenicity of Five Strains of Toxoplasma gondii from Different Animals to Chickens
Parasites, Hosts and Diseases
2015 .04
-
Comparison of Antibody Response Elicited by ChAdOx1 and BNT162b2 COVID-19 Vaccine
Journal of Korean Medical Science
2021 .11
-
Evolution, global spread, and pathogenicity of highly pathogenic avian influenza H5Nx clade 2.3.4.4
Journal of Veterinary Science
2017 .08
-
무증상, 경증 또는 중등증 코로나19 감염 후 백신 접종자와 비접종자의 뉴클레오캡시드 단백질 항체 생산 비교
의료관련감염관리
2023 .06
-
Diagnosis of Balamuthia mandrillaris Encephalitis by Thymine–Adenine Cloning Using Universal Eukaryotic Primers
Annals of Laboratory Medicine
2022 .03
-
광주광역시 요양시설 코로나19 집단 발생 사례에서 코로나19 백신 접종에 의한 사망 예방 효과
농촌의학·지역보건
2022 .06
-
Validation of Pathogenicity of Gene Variants in Fanconi Anemia Using Patient-derived Dermal Fibroblasts
Annals of Laboratory Medicine
2023 .01
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-151-25-02-092180598