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논문 기본 정보

자료유형
학술저널
저자정보
Sharif Fahimeh (Department of Microbiology, Islamic Azad University Qom Branch, Qom, Iran.) Nazari Razieh (Department of Microbiology, Islamic Azad University Qom Branch, Qom, Iran.) Fasihi-Ramandi Mahdi (Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.) Taheri Ramezan Ali (Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.) Zargar Mohsen (Department of Microbiology, Islamic Azad University Qom Branch, Qom, Iran.)
저널정보
대한백신학회 Clinical and Experimental Vaccine Research Clinical and Experimental Vaccine Research Vol.13 No.3
발행연도
2024.7
수록면
232 - 241 (10page)
DOI
10.7774/cevr.2024.13.3.232

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Purpose: Brucellosis, a zoonotic infectious disease, is a worldwide health issue affecting animals and humans. No effective human vaccine and the complications caused by the use of animal vaccines are among the factors that have prevented the eradication of the disease worldwide. However, bio-engineering technologies have paved the way for designing new targeted and highly efficacious vaccines. In this regard, the study aimed to evaluate immunity induced by mannosylated niosome containing Brucella recombinant trigger factor/Bp26/ Omp31 (rTBO) chimeric protein in a mouse model. Materials and Methods: rTBO as chimeric antigen (Ag) was expressed in Escherichia coli BL21 (DE3) and, after purification, loaded on niosome and mannosylated niosome. The characteristics of the nanoparticles were assessed. The mice were immunized using rTBO, niosome, and mannosylated niosome-rTBO in intranasal and intraperitoneal routes. Serum antibodies (immunoglobulin [Ig]A, IgG, IgG1, and IgG2a) and splenocyte cytokines (interferon gamma, interleukin [IL]-4, and IL-12) were evaluated in immunized mice. Finally, immunized mice were challenged by B. melitensis and B. abortus. A high antibody level was produced by niosomal antigen (Nio-Ag) and mannosylated noisomal antigen (Nio-Man-Ag) compared to the control after 10, 24, and 38 days of immunization. The IgG2a/IgG1 titer ratio for Nio-Man-Ag was 1.2 and 1.1 in intraperitoneal and intranasal methods and lower than one in free Ag and Nio-Ag. Cytokine production was significantly higher in the immunized animal with Ag-loaded nanoparticles than in the negative control group (p<0.05). Moreover, cytokine and antibody levels were significantly higher in the injection than in the inhalation method (p<0.05). Results: The combination of mannosylated noisome and rTBO chimeric proteins stimulate the cellular and humoral immune response and produce cytokines, playing a role in developing the protective acquired immune response in the Brucella infectious model. Also, the intraperi toneal route resulted in a successful enhancement of cytokines production more than intrana sal administration. Conclusion: Designing an effective vaccine candidate against Brucella that selectively induces cellular and humoral immune response can be done by selecting a suitable nanonio some formulation as an immunoadjuvant and recombinant protein as an immune response stimulating Ag.
#Brucellosis #Mannose #Niosome #Vaccine #Recombinant proteins

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