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논문 기본 정보

자료유형
학술저널
저자정보
Ghazal karevan (Nourdanesh Institute of Higher Education)
저널정보
대한백신학회 Clinical and Experimental Vaccine Research Clinical and Experimental Vaccine Research Vol.10 No.1
발행연도
2021.1
수록면
35 - 43 (9page)

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Purpose: Brucellosis as a worldwide zoonotic illness affect domestic animals and humans doesn’t have any vaccine for the prevention of infection in humans yet. The aim of this study was to evaluate the specific immune response following the administration of glycine nanoparticles as adjuvant and delivery system of a chimeric antigen contained trigger factor, Omp31, and Bp26 in murine model. Materials and Methods: The chimeric antigen of Brucella was cloned and expressed in Escherichia coli (E. coli) BL21 (DE3). Purification and characterization of recombinant protein was conducted through Ni-NTA (nickel-nitrilotriacetic acid) agarose, SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), and Western blot. Nanoparticle characteristics including morphology, particle size distribution, zeta potential, protein retention rate, and release rate were measured in vitro. Subsequently, nanoparticle contained antigen was administered to mice and blood sample was taken to measured the antibody level. Results: The protein retention in the nanoparticles was successfully done and the nanoparticle characteristics were appropriate. The average size of glycine particles containing antigen was about 174 nm, and the absorption of protein was approximately 61.27% of the initial value, with a release rate of approximately 70% after 8 hours. Enzyme-linked immunosorbent assay result proved that the immunized sera of mice which were administered with nano-formula contains high levels of antibodies (immunoglobulin G) against recombinant chimeric antigen and also a high level of mucosal antibody (immunoglobulin A) in the oral group, which showed a desirable immunity against Brucella. Conclusion: The results showed that chimeric antigen-loaded glycine nanoparticles can act as a vaccine candidate for inducing the cellular and humoral immune response against brucellosis.
#Nanoparticles #Vaccine #Brucellosis #Glycine #Chimeric antigens

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참고문헌 (33)

참고문헌 신청
Abkar M / 2018 / Intraperitoneal immunization with Urease loaded N-trimethyl chitosan nanoparticles elicits high protection against Brucella melitensis and Brucella abortus infections / Immunol Lett 199:53~60 google schola Moravej H / 2019 / Cytotoxicity and antibacterial effect of Trp-substituted CM11 cationic peptide against drug-resistant isolates of Brucella melitensis alone and in combination with recommended antibiotics / Int J Pept Res Ther 25:235~245 google schola Abkar M / 2017 / Oral immunization of mice with Omp31-loaded N-trimethyl chitosan nanoparticles induces high protection against Brucella melitensis infection / Int J Nanomedicine 12:8769~8778 google schola Mohammadi Azad Z / 2017 / In vitro synergistic effects of a short cationic peptide and clinically used antibiotics against drug-resistant isolates of Brucella melitensis / J Med Microbiol 66:919~926 google schola Abdollahi A / 2016 / Immunoreactivity evaluation of a new recombinant chimeric protein against Brucella in the murine model / Iran J Microbiol 8:193~202 google schola

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