지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.12
- 수록면
- 157 - 162 (6page)
- DOI
- 10.13041/jpvm.2018.42.4.157
이용수
초록· 키워드
오류제보하기
This study evaluated the protective effects of a combination of eight B. abortus recombinant proteins that were cloned and expressed into a pMal vector system and DH5α: nucleoside diphosphate kinase (rNdk), 50S ribosomal protein (rL7/L12), malate dehydrogenase (rMDH), DNA starvation/stationary phase protection protein (rDps), elongation factor (rTsf), arginase (rRocF), superoxide dismutase (rSodC), and riboflavin synthase subunit beta (rRibH). The proteins were induced, purified, and administered intraperitoneally into BALB/c mice. The mice were immunized three times at weeks 0, 2, and 5 and then infected intraperitoneally (IP) with 5×104 CFU of virulent B. abortus 544 one week after the last immunization. The spleens were collected and the bacterial burden was evaluated at four weeks post-infection. The results showed that this combination produced a significant reduction of the bacterial burden in the spleen with a log reduction of 1.01 compared to the PBS group. Cytokine analysis revealed induction of the cell-mediated immune response in that TNF (tumor necrosis factor) and proinflammatory cytokines IL-6 (Interleukin 6) and MCP-1 (macrophage chemoattractant protein-1) were elevated significantly. In summary, vaccination with a combination of eight different proteins induced a significant protective effect indicative of a cell mediated immune response.
목차
등록된 정보가 없습니다.
참고문헌 (16)
참고문헌 신청
Baldwin CL / 1993 / Macrophage control of Brucella abortus: influence of cytokines and iron / Trends Microbiol 1:99~104
Corbel MJ / 1997 / Brucellosis: an overview / Emerg Infect Dis 3:213~221
Dubray G / 1980 / Isolation of three Brucella abortus cell wall antigens protective in murine experimental brucellosis / Ann Rech Vet 11:367~373
Hop HT / 2018 / Immunization of BALB/c mice with a combination of four recombinant Brucella abortus proteins, AspC, Dps, InpB and Ndk, confers a marked protection against a virulent strain of Brucella abortus / Vaccine 36:3027~3033
Hop HT / 2015 / Immunogenicity and protective effect of recombinant Brucella abortus Ndk (rNdk) against a virulent strain Brucella abortus 544 infection in BALB/c mice / FEMS Microbiol Lett 362:1~6
Corbel MJ / 1997 / Brucellosis: an overview / Emerg Infect Dis 3:213~221
Dubray G / 1980 / Isolation of three Brucella abortus cell wall antigens protective in murine experimental brucellosis / Ann Rech Vet 11:367~373
Hop HT / 2018 / Immunization of BALB/c mice with a combination of four recombinant Brucella abortus proteins, AspC, Dps, InpB and Ndk, confers a marked protection against a virulent strain of Brucella abortus / Vaccine 36:3027~3033
Hop HT / 2015 / Immunogenicity and protective effect of recombinant Brucella abortus Ndk (rNdk) against a virulent strain Brucella abortus 544 infection in BALB/c mice / FEMS Microbiol Lett 362:1~6
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