지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Subunit vaccines are being developed as a potential therapy for preventing microbial pathogen infection. In this study, the immunogenicity of recombinant Brucella (B.) abortus Fe/Mn superoxide dismutase (rFe/Mn SOD) protein as a subunit vaccine against B. abortus was investigated in BALB/c mice model. Brucella Fe/Mn SOD gene was cloned into a pcold-TF DNA vector. The bacterial recombinant protein was expressed using the Escherichia coli DH5α strain with a size of 82.50 kDa. The western blotting assay showed that rFe/Mn SOD reacted with Brucella-positive serum, indicating the potential immunoreactivity of this recombinant protein. After the second and third vaccinations, the peripheral CD4+ T cell population was increased significantly in the rFe/Mn SOD-immunized mice group compared to the PBS control group. Moreover, immunization of this recombinant protein increased the CD4+ T cell population from the first vaccination to the third vaccination. Meanwhile, the CD8+ T cells were slightly enhanced after the second vaccination compared to the first vaccination and compared to control groups.
Fourteen days after the bacterial infection, the splenomegaly and the number of bacteria in the spleen were evaluated. The result showed that both rFe/Mn SOD and positive control RB51 decreased the bacterial replication in the spleen and the splenomegaly compared to control groups. Altogether, these results suggested that rFe/Mn SOD could induce host immunity against B. abortus infection.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Immunization with Brucella abortus recombinant proteins protects BALB/c mice from Brucella abortus 544 infection
예방수의학회지
2018 .12
-
Immunization of a combination of four recombinant Brucella abortus proteins Adk, Ndk, L7/L12 and SecB induced protective immune responses in BALB/c mice against a virulent strain B. abortus 544 infection
예방수의학회지
2020 .01
-
Vaccination of 50S ribosomal protein L27 decreased susceptibility to Brucella abortus 544 infection in BALB/c mice
예방수의학회지
2019 .01
-
Resistance to Brucella abortus 544 challenge in BALB/c mice by inoculation of highly immunogenic multiple B. abortus antigens
예방수의학회지
2019 .01
-
Vaccination of goats with a combination Salmonella vector expressing four Brucella antigens (BLS, PrpA, Omp19, and SOD) confers protection against Brucella abortus infection
Journal of Veterinary Science
2018 .09
-
Protection of palmitic acid treatment in RAW264.7 cells and BALB/c mice during Brucella abortus 544 infection
Journal of Veterinary Science
2021 .03
-
Succinic acid inhibits the uptake of Brucella abortus 544 into RAW 264.7 cells and promotes survival of B. abortus in ICR mice
예방수의학회지
2021 .12
-
Isolation and characterization of Brucella abortus isolates from wildlife species in South Korea
Korean Journal of Veterinary Research(대한수의학회지)
2016 .10
-
The in vitro effects of synthetic complement peptide C3a during Brucella abortus 544 infection in a murine professional phagocyte RAW264.7 cell line
예방수의학회지
2021 .12
-
Effect of Manganese Content on the Magnetic Susceptibility of Ferrous-Manganese Alloys: Correlation between Microstructure on X-Ray Diffraction and Size of the Low-Intensity Area on MRI
Investigative Magnetic Resonance Imaging
2015 .01
-
Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein
Journal of Veterinary Science
2016 .03
-
Inhibitory effect of red ginseng acidic polysaccharide from Korean red ginseng on phagocytic activity and intracellular replication of Brucella abortus in RAW 264.7 cells
Journal of Veterinary Science
2016 .09
-
Molecular characteristics of Brucella abortus mutants generated using EZ-Tn5Tm pMODTm-3 transposon system
예방수의학회지
2015 .12
-
Different invasion efficiencies of Brucella abortus wild-type and mutants in RAW 264.7 and THP-1 phagocytic cells and HeLa non-phagocytic cells
Korean Journal of Veterinary Research(대한수의학회지)
2018 .07
-
Analysis of protein expression in mutants with different growth rates by two-dimensional gel electrophoresis and LC-MS/MS peptide analysis
Journal of Veterinary Science
2018 .03
-
Protective efficacy of attenuated Salmonella Typhimurium strain expressing BLS, Omp19, PrpA, or SOD of Brucella abortus in goats
Journal of Veterinary Science
2021 .03
-
A Novel PCR Assay for Detecting Brucella abortus and Brucella melitensis
Osong Public Health and Research Persptectives
2017 .01
-
Effect of immunization routes and protective efficacy of Brucella antigens delivered via Salmonella vector vaccine
Journal of Veterinary Science
2018 .05
-
Tannic acid-mediated immune activation attenuates infection in mice
Journal of Veterinary Science
2018 .01
-
Nucleomodulin BspJ as an effector promotes the colonization of Brucella abortus in the host
Journal of Veterinary Science
2022 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0