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학제간연구 과학기술학 뇌인지과학 기술정책 기타 복합학

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논문 기본 정보

자료유형
학술저널
저자정보
Jia-Qi Chu (Affiliated Hospital of Guangdong Medical University) Shuai Huang (Affiliated Hospital of Guangdong Medical University) Xuan-Yan Fan (Affiliated Hospital of Guangdong Medical University) Wei Ye (Affiliated Hospital of Guangdong Medical University) Rui Huang (Affiliated Hospital of Guangdong Medical University) Shi-Cai Ye (Affiliated Hospital of Guangdong Medical University) Cai-Yuan Yu (Affiliated Hospital of Guangdong Medical University) Wei-Yun Wu (Affiliated Hospital of Guangdong Medical University) Yu Zhou (Affiliated Hospital of Guangdong Medical University) Wei Zhou (Taishan Medical College) Young-Ha Lee (Chungnam National University) Juan-Hua Quan (Affiliated Hospital of Guangdong Medical University)
저널정보
대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.56 No.4
발행연도
2018.8
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325 - 334 (10page)

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Toxoplasma gondii is an apicomplexan zoonotic protozoan parasite that infects most species of warm-blooded animals, including humans. The heavy incidence and severe or lethal damage caused by T. gondii infection clearly indicate a need for the development of an effective vaccine. T. gondii GRA8 is a member of the dense granules protein family and is used as a marker of acute infection. In the present study, we evaluated the protective immunity induced by DNA vaccination based on a recombinant eukaryotic plasmid, pDsRed2-GRA8, against acute toxoplasmosis in mice. BALB/c mice were intramuscularly immunized with the pDsRed2-GRA8 plasmid and then challenged by infection with the highly virulent GFP-RH strain of T. gondii. The specific immune responses and protective efficacy against T. gondii of this vaccine were analyzed by measuring cytokine and serum antibody titers, splenocyte proliferation assays, and the survival times of mice after challenge. Our results showed that mice immunized with pDsRed2-GRA8 demonstrated specific humoral and cellular responses, induced higher IgG antibody titers with predominant IgG2a production; increased levels of IL-10, IL-12 (p70), IFN-γ, TNF-α, and splenocyte proliferation; and prolonged survival times compared to those of control mice. The present study showed that DNA immunization with pDsRed2-GRA8 induced humoral and cellular immune responses, and all immunized mice showed greater Th1-type immune responses and longer survival times than those of control mice. These results indicated that T. gondii GRA8 DNA immunization induces a partial protective effect against acute toxoplasmosis.
#Toxoplasma gondii #DNA vaccine #GRA8 #immune response

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

참고문헌 (44)

참고문헌 신청
Dubey JP / 2008 / The history of Toxoplasma gondii-the first 100 years / J Eukaryot Microbiol 55:467~475 Crossref Dubey JP / 2011 / High prevalence and genotypes of Toxoplasma gondii isolated from goats, from a retail meat store, destined for human consumption in the USA / Int J Parasitol 41:827~833 Crossref Nissapatorn V / 2004 / Toxoplasmosis in HIV/AIDS patients: a current situation / Jpn J Infect Dis 57:160~165 google schola Thomas F / 2012 / Incidence of adult brain cancers is higher in countries where the protozoan parasite Toxoplasma gondii is common / Biol Lett 8:101~103 Crossref Lebech M / 1999 / Feasibility of neonatal screening for toxoplasma infection in the absence of prenatal treatment / Lancet 353:1834~1837 Crossref

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