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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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초록· 키워드
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This study investigated the efficacy of four Brucella (B.) abortus recombinant proteins, namely adenylate kinase (Adk), nucleoside diphosphate kinase (Ndk), 50S ribosomal protein (L7/L12) and preprotein translocase subunit (SecB), as a combined subunit vaccine (CSV) against B. abortus infection in BALB/c mice. Immunoblotting assay showed that these four recombinant proteins as well as pcold-TF vector reacted individually with Brucella-positive serum, but not with Brucella-negative serum. The peripheral blood CD4+ T cell population was increased in CSV-immunized mice compared to PBS and pcold-TF vector groups. In addition, CSV and pcold-TF groups displayed induced IgG1 and IgG2a antibodies production compared to PBS and RB51 group, whereas IgG2a titer was higher than IgG1 titer in CSV group. The secretion profiles of IgG1 and IgG2a production together with an enhancement of CD4+ T cell population suggested that CSV did not only induce T helper 1 (Th1) T cell immunity but also humoral immunity. Therein, Th1 T cell immunity is more predominant in eliminating intracellular bacteria B. abortus. Furthermore, CSV immunization significantly reduced the bacterial burden in the spleen as well as the spleen weight in comparison to PBS and pcold-TF groups. Altogether, combination of these antigens could be potential to induce protective immunity against B. abortus infection in animals.
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참고문헌 (21)
참고문헌 신청
Lauren Togonon Arayan / 2019 / Substantial Protective Immunity Conferred by a Combination of Brucella abortus Recombinant Proteins against Brucella abortus 544 Infection in BALB/c Mice / Journal of Microbiology and Biotechnology 29(2):330~338
Cassataro J / 2005 / Vaccination with the recombinant Brucella outer membrane protein 31 or a derived 27-amino-acid synthetic peptide elicits a CD4+ T helper 1 response that protects against Brucella melitensis infection / Infect Immun 73:8079~8088
Chopra P / 2004 / Nucleoside diphosphate kinase of Mycobacterium tuberculosis acts as GTPase-activating protein for Rho-GTPases / FEBS Lett 571:212~216
de Figueiredo P / 2015 / Pathogenesis and immunobiology of Brucellosis. Review of Brucella-Host interactions / Am J Clin Pathol 185:1505~1517
Dorneles EMS / 2015 / Immune response triggered by Brucella abortus following infection or vaccination / Vaccine 33:3659~3666
Cassataro J / 2005 / Vaccination with the recombinant Brucella outer membrane protein 31 or a derived 27-amino-acid synthetic peptide elicits a CD4+ T helper 1 response that protects against Brucella melitensis infection / Infect Immun 73:8079~8088
Chopra P / 2004 / Nucleoside diphosphate kinase of Mycobacterium tuberculosis acts as GTPase-activating protein for Rho-GTPases / FEBS Lett 571:212~216
de Figueiredo P / 2015 / Pathogenesis and immunobiology of Brucellosis. Review of Brucella-Host interactions / Am J Clin Pathol 185:1505~1517
Dorneles EMS / 2015 / Immune response triggered by Brucella abortus following infection or vaccination / Vaccine 33:3659~3666
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