지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
In this study, we examined the protective immunity of a combination of seven Brucella abortus recombinant proteins; superoxide dismutase (rSodC), riboflavin synthase subunit beta (rRibH), 50S ribosomal protein (50s rL7/L12), nucleoside diphosphate kinase (rNdk), malate dehydrogenase (rMDH), arginase (rRocF), and elongation factor (rTsf) cloned in a pMal vector system and expressed in DH5α. Mice groups were immunized thrice with a combined subunit vaccine (CSV-7) at 0, 2, and 5 weeks and subsequently challenged with B. abortus at 5 × 104 CFU at 6 weeks. At four weeks post-infection, the mice were sacrificed and the bacterial burden in their spleens was quantified. Results revealed bacterial log reductions of 0.63 and 0.34 in comparison to PBS and maltose-binding protein (MBP), respectively. Cytokine profiling revealed a marked increase in IFN-γ (interferon-gamma), MCP-1 (macrophage chemoattractant protein-1) and IL-6 (interleukin 6) cytokines at 5-weeks post-immunization. On the other hand, only TNF was heightened at 7-weeks post-immunization. In general, this cytokine profile is consistently reflective of a Th1 immune response, which is beneficial for host immunoresistance.
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등록된 정보가 없습니다.
참고문헌 (15)
참고문헌 신청
Al-Mariri A / 2001 / Protection of BALB/c mice against Brucella abortus 544 challenge by vaccination with bacterioferritin or P39 recombinant protein with CpG oligodeoxynucleotides as adjuvant / Infect Immun 69:4816~4822
Lauren Togonon Arayan / 2018 / Immunization with Brucella abortus recombinant proteins protects BALB/c mice from Brucella abortus 544 infection / 예방수의학회지 42(4):157~162
Lauren Togonon Arayan / 2019 / Substantial Protective Immunity Conferred by a Combination of Brucella abortus Recombinant Proteins against Brucella abortus 544 Infection in BALB/c Mice / Journal of Microbiology and Biotechnology 29(2):330~338
Baldwin CL / 2006 / Host immune responses to the intracellular bacterium Brucella: does the bacterium instruct the host to facilitate chronic infection? / Crit Rev Immunol 26:407~442
Ewalt DR / 1994 / Characteristics of a Brucella species from a bottle-nosed dolphin (Tursiops truncatus) / J Vet Diagn Invest 6:448~452
Lauren Togonon Arayan / 2018 / Immunization with Brucella abortus recombinant proteins protects BALB/c mice from Brucella abortus 544 infection / 예방수의학회지 42(4):157~162
Lauren Togonon Arayan / 2019 / Substantial Protective Immunity Conferred by a Combination of Brucella abortus Recombinant Proteins against Brucella abortus 544 Infection in BALB/c Mice / Journal of Microbiology and Biotechnology 29(2):330~338
Baldwin CL / 2006 / Host immune responses to the intracellular bacterium Brucella: does the bacterium instruct the host to facilitate chronic infection? / Crit Rev Immunol 26:407~442
Ewalt DR / 1994 / Characteristics of a Brucella species from a bottle-nosed dolphin (Tursiops truncatus) / J Vet Diagn Invest 6:448~452
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