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논문 기본 정보

자료유형
학술저널
저자정보
저널정보
대한백신학회 Clinical and Experimental Vaccine Research Clinical and Experimental Vaccine Research Vol.10 No.2
발행연도
2021.1
수록면
106 - 122 (17page)

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Purpose: In the present study, meningococcal serogroup B outer membrane vesicles (OMVs) were associated with bilayer fragments of a cationic lipid, dioctadecyldimethylammonium (DDA-BF), used as adjuvant, in an antigenic preparation tested in adult female outbred mice. This adjuvant was compared to the traditional adjuvant aluminum hydroxide. Materials and Methods: The potential in generating humoral response was evaluated by enzyme-linked immunosorbent assay (ELISA). Individual serum was collected and immunoglobulin G (IgG), IgG1, IgG2a, and IgG2b were quantified. Analyses were carried out 15 and 60 days after immunization. Antibodies avidity index were also analyzed by ELISA. Immunoblot and dot-ELISA were carried out to evaluate specific reaction for homologous strains and cross-reactive antigens present in other meningococcal strains isolated in 2011–2012 year, in Brazil. Delayed type hypersensitivity was used as indicative of cellular immunity and compared between two experimental groups, 24 hours after homologous strain challenge. Results: The OMVs of Neisseria meningitidis, and N. lactamica (related species) were characterized by electrophoretic separation of proteins in 13% polyacrylamide gel. The strains presented antigens in the range of 8 to 130 kDa, showing a heterogeneous protein migration pattern. In the group immunized with OMVs/DDA-BF, we found no significant production of total IgG 15 days after the first immunization. On the other hand, 60 days after first immunization both adjuvants act benefiting total IgG production similarly. The antibodies of the IgG isotype produced by animals immunized after one or two doses after first immunization, showed intermediate and high avidity, independent on the adjuvant used. In both experimental groups the swelling of the footpads was significantly higher than those of the controls, suggesting that only one dose was enough to stimulate the generation of cellular immunity. Conclusion: The use of this cationic adjuvant for N. meningitidis OMVs preparation revealed good potential for future new antigen preparation for N. meningitidis vaccine.
#Neisseria meningitidis #Dioctadecyldimethylammonium bromide #Bilayer fragments #Outer membrane vesicles #Immune response in outbred mouse

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참고문헌 (48)

참고문헌 신청
Aucouturier J / 2002 / Montanide ISA 720 and 51 : a new generation of water in oil emulsions as adjuvants for human vaccines / Expert Rev Vaccines 1:111~118 google schola Guy B / 2007 / The perfect mix : recent progress in adjuvant research / Nat Rev Microbiol 5:505~517 google schola Holst J / 2009 / Properties and clinical performance of vaccines containing outer membrane vesicles from Neisseria meningitidis / Vaccine 27(Suppl 2):B3~B12 google schola Rappuoli R / 2001 / Reverse vaccinology, a genome-based approach to vaccine development / Vaccine 19:2688~2691 google schola Masignani V / 2003 / Vaccination against Neisseria meningitidis using three variants of the lipoprotein GNA1870 / J Exp Med 197:789~799 google schola

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