지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.3
- 수록면
- 1 - 13 (13page)
이용수
초록· 키워드
오류제보하기
Background: Attenuated Salmonella strain can be used as a vector to transport immunogens to the host antigen-binding sites.
Objectives: The study aimed to determine the protective efficacy of attenuated Salmonella strain expressing highly conserved Brucella immunogens in goats.
Methods: Goats were vaccinated with Salmonella vector expressing individually lipoprotein outer-membrane protein 19 (Omp19), Brucella lumazine synthase (BLS), proline racemase subunit A (PrpA), Cu/Zn superoxide dismutase (SOD) at 5 × 10<SUP>9</SUP> CFU/mL and challenge of all groups was done at 6 weeks after vaccination.
Results: Among these vaccines inoculated at 5 × 10<SUP>9</SUP> CFU/mL in 1 mL, Omp19 or SOD showed significantly higher serum immunoglobulin G titers at (2, 4, and 6) weeks post-vaccination, compared to the vector control. Interferon-γ production in response to individual antigens was significantly higher in SOD, Omp19, PrpA, and BLS individual groups, compared to that in the vector control (all p < 0.05). Brucella colonization rate at 8 weeks post-challenge showed that most vaccine-treated groups exhibited significantly increased protection by demonstrating reduced numbers of Brucella in tissues collected from vaccinated groups. Real-time polymerase chain reaction revealed that Brucella antigen expression levels were reduced in the spleen, kidney, and parotid lymph node of vaccinated goats, compared to the nonvaccinated goats. Besides, treatment with vaccine expressing individual antigens ameliorated brucellosis-related histopathological lesions.
Conclusions: These results delineated that BLS, Omp19, PrpA, and SOD proteins achieved a definite level of protection, indicating that Salmonella Typhimurium successfully delivered Brucella antigens, and that individual vaccines could differentially elicit an antigen-specific immune response.
Objectives: The study aimed to determine the protective efficacy of attenuated Salmonella strain expressing highly conserved Brucella immunogens in goats.
Methods: Goats were vaccinated with Salmonella vector expressing individually lipoprotein outer-membrane protein 19 (Omp19), Brucella lumazine synthase (BLS), proline racemase subunit A (PrpA), Cu/Zn superoxide dismutase (SOD) at 5 × 10<SUP>9</SUP> CFU/mL and challenge of all groups was done at 6 weeks after vaccination.
Results: Among these vaccines inoculated at 5 × 10<SUP>9</SUP> CFU/mL in 1 mL, Omp19 or SOD showed significantly higher serum immunoglobulin G titers at (2, 4, and 6) weeks post-vaccination, compared to the vector control. Interferon-γ production in response to individual antigens was significantly higher in SOD, Omp19, PrpA, and BLS individual groups, compared to that in the vector control (all p < 0.05). Brucella colonization rate at 8 weeks post-challenge showed that most vaccine-treated groups exhibited significantly increased protection by demonstrating reduced numbers of Brucella in tissues collected from vaccinated groups. Real-time polymerase chain reaction revealed that Brucella antigen expression levels were reduced in the spleen, kidney, and parotid lymph node of vaccinated goats, compared to the nonvaccinated goats. Besides, treatment with vaccine expressing individual antigens ameliorated brucellosis-related histopathological lesions.
Conclusions: These results delineated that BLS, Omp19, PrpA, and SOD proteins achieved a definite level of protection, indicating that Salmonella Typhimurium successfully delivered Brucella antigens, and that individual vaccines could differentially elicit an antigen-specific immune response.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Vaccination of goats with a combination Salmonella vector expressing four Brucella antigens (BLS, PrpA, Omp19, and SOD) confers protection against Brucella abortus infection
Journal of Veterinary Science
2018 .09
-
Effect of immunization routes and protective efficacy of Brucella antigens delivered via Salmonella vector vaccine
Journal of Veterinary Science
2018 .05
-
Development and trial of vaccines against Brucella
Journal of Veterinary Science
2017 .08
-
The two highly immunogenic antigens of Brucella: lipopolysaccharide (LPS) and outer membrane proteins (OMPs)
예방수의학회지
2015 .12
-
Isolation and characterization of Brucella abortus isolates from wildlife species in South Korea
Korean Journal of Veterinary Research(대한수의학회지)
2016 .10
-
Immunogenicity of glycine nanoparticles containing a chimeric antigen as Brucella vaccine candidate
Clinical and Experimental Vaccine Research
2021 .01
-
Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein
Journal of Veterinary Science
2016 .03
-
Nucleomodulin BspJ as an effector promotes the colonization of Brucella abortus in the host
Journal of Veterinary Science
2022 .01
-
Protective effects of recombinant Brucella abortus protein Fe/Mn superoxide dismutase against a virulent strain B. abortus 544 infection in BALB/c mice
예방수의학회지
2022 .12
-
Outer membrane proteins of Salmonella Typhimurium as an adjuvant in rabies vaccine
Clinical and Experimental Vaccine Research
2021 .01
-
Comparative proteomic analysis of outer membrane protein 43 ( omp43)-deficient Bartonella henselae
Journal of Veterinary Science
2018 .01
-
A Novel PCR Assay for Detecting Brucella abortus and Brucella melitensis
Osong Public Health and Research Persptectives
2017 .01
-
The in vitro effects of synthetic complement peptide C3a during Brucella abortus 544 infection in a murine professional phagocyte RAW264.7 cell line
예방수의학회지
2021 .12
-
Immune modulatory and protective effects of Brucella abortus cellular antigens, arginase and elongation factor Ts, as subunit vaccines against murine brucellosis
예방수의학회지
2018 .06
-
Intranasal and intraperitoneal immunization against Brucella infection using niosome and mannosylated niosomes containing Brucella recombinant trigger factor/Bp26/Omp31 chimeric protein in a mouse model
Clinical and Experimental Vaccine Research
2024 .07
-
Reverse vaccinology approach for the identification and characterization of outer membrane proteins of Shigella flexneri as potential cellular- and antibody-dependent vaccine candidates
Clinical and Experimental Vaccine Research
2020 .01
-
Resistance to Brucella abortus 544 challenge in BALB/c mice by inoculation of highly immunogenic multiple B. abortus antigens
예방수의학회지
2019 .01
-
Succinic acid inhibits the uptake of Brucella abortus 544 into RAW 264.7 cells and promotes survival of B. abortus in ICR mice
예방수의학회지
2021 .12
-
Lipid-based antigen delivery systems
Journal of Pharmaceutical Investigation
2016 .07
-
Vaccination of 50S ribosomal protein L27 decreased susceptibility to Brucella abortus 544 infection in BALB/c mice
예방수의학회지
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2023-528-001326113