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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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We investigated the effect of a synthetic complement peptide C3a on the outcome of Brucella abortus 544 infection in a murine macrophage cell line RAW264.7 cell. First, we determined the highest non-cytotoxic concentration of the peptide in the cell line. We also found that the peptide significantly increased the growth of the bacteria at 8 and 24 h. Although the number of bacterial CFU was also elevated at 48 and 72 h, the increases were not significant as compared to controls. We further investigated the effect of C3a peptide on the growth of Brucella by pre-incubating the peptide at various temperatures and found that the effect was reversed at 24 h post-incubation suggesting that incubation of peptide at high temperatures including 65°C or 95°C could inactivate its action. This also could indicate the beneficial effect of high temperature during infection. Although several studies reported the inhibitory effect of different antimicrobial peptides including C3a, the present study preliminarily revealed that it had no positive contribution on the control of B. abortus 544 infection in vitro and indirectly to its receptor, CD88, which belongs to GPCR. Moreover, the encouraged further exploration of the effect of other similar peptides would be performed for the purpose of finding Brucella-host cell interaction for the control of disease progression.
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참고문헌 (16)
참고문헌 신청
Ahmed W / 2016 / Establishment of chronic infection : Brucella's stealth strategy / Front Cell Infect Microbiol 6:30
Lauren Togonon Arayan / 2017 / The Bactericidal Effect of High Temperature Is an Essential Resistance Mechanism of Chicken Macrophage against Brucella abortus Infection / Journal of Microbiology and Biotechnology 27(10):1837~1843
Dadar M / 2021 / Importance of brucellosis control programs of livestock on the improvement of one health / Vet Q 41:137~151
DiScipio RG / 2007 / The role of the complement anaphylatoxins in the recruitment of eosinophils / Int Immunopharmacol 7(14):1909~1923
Dutow P / 2014 / The complement C3a receptor is critical in defense against Chlamydia psittaci in mouse lung infection and required for antibody and optimal T cell response / J Infect Dis 209(8):1269~1278
Lauren Togonon Arayan / 2017 / The Bactericidal Effect of High Temperature Is an Essential Resistance Mechanism of Chicken Macrophage against Brucella abortus Infection / Journal of Microbiology and Biotechnology 27(10):1837~1843
Dadar M / 2021 / Importance of brucellosis control programs of livestock on the improvement of one health / Vet Q 41:137~151
DiScipio RG / 2007 / The role of the complement anaphylatoxins in the recruitment of eosinophils / Int Immunopharmacol 7(14):1909~1923
Dutow P / 2014 / The complement C3a receptor is critical in defense against Chlamydia psittaci in mouse lung infection and required for antibody and optimal T cell response / J Infect Dis 209(8):1269~1278
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