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This study aims to investigate the effects of exogenous succinic acid (SCA) on Brucella (B.) abortus infection in macrophage RAW 264.7 cells and ICR mice. Firstly, the in vitro experiment was conducted by MTT cytotoxicity and bacterial internalization assay to evaluate the uptake of B. abortus into macrophage cells. Two non-cytotoxic concentrations of SCA demonstrated attenuated invasion of Brucella into macrophages at 30 and 45 min post- infection (pi). Secondly, ICR mice were treated with SCA and infected with B. abortus. On day-14 pi, spleen and blood serum were collected to evaluate the bacterial burden and total spleen weight as well as the production of cytokine/chemokine, respectively. The results showed that SCA treatment promoted bacterial growth and reduced the total spleen weight in mice. Furthermore, SCA treatment increased the level of IL-10 cytokine in the sera, while dampening the production of MCP-1 chemokine compared to the control. The results of bacterial load in spleen and spleen weight together with cytokine/chemokine production profile in the sera indicated that SCA induced the host anti-inflammatory response which is beneficial for the survival of Brucella. Therefore, these findings suggest that SCA contributed to host immunity against Brucella infection and the emerging potential topic-immunometabolism should be invested for further investigations.
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참고문헌 (21)
참고문헌 신청
Berry LJ / 1953 / The relation of the Tricarboxylic acid cycle to bacterial infection II. The effect of fluoroacetate, arsenite, citrate, and succinate on Salmonella typhimurium infections in mice / J Infect Dis 93:83~92
Jean Celli / 2006 / Surviving inside a macrophage: The many ways of Brucella / Research in Microbiology 157(2):93~98
P. Conti / 2001 / MCP-1 and RANTES Are Mediators of Acute and Chronic Inflammation / Allergy and Asthma Proceedings 22(3):133~137
Paul de Figueiredo / 2015 / Pathogenesis and Immunobiology of Brucellosis / The American Journal of Pathology 185(6):1505~1517
D M Fernandes / 1995 / Interleukin-10 downregulates protective immunity to Brucella abortus / Infection and Immunity 63(3):1130~1133
Jean Celli / 2006 / Surviving inside a macrophage: The many ways of Brucella / Research in Microbiology 157(2):93~98
P. Conti / 2001 / MCP-1 and RANTES Are Mediators of Acute and Chronic Inflammation / Allergy and Asthma Proceedings 22(3):133~137
Paul de Figueiredo / 2015 / Pathogenesis and Immunobiology of Brucellosis / The American Journal of Pathology 185(6):1505~1517
D M Fernandes / 1995 / Interleukin-10 downregulates protective immunity to Brucella abortus / Infection and Immunity 63(3):1130~1133
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