Reverse vaccinology approach for the identification and characterization of outer membrane proteins of Shigella flexneri as potential cellular- and antibody-dependent vaccine candidates

Chiuan Yee Leow; Ada Kazi; Che Muhammad Khairul Hisyam Ismail; Candy Chuah; Boon Huat Lim; Chiuan Herng Leow; Kirnpal Kaur Banga Singh

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자료유형: 학술저널

저자정보: Chiuan Yee Leow (Universiti Sains Malaysia) Ada Kazi (Universiti Sains Malaysia) Che Muhammad Khairul Hisyam Ismail (Universiti Sains Malaysia) Candy Chuah (Universiti Sains Malaysia) Boon Huat Lim (Universiti Sains Malaysia) Chiuan Herng Leow (Universiti Sains Malaysia) Kirnpal Kaur Banga Singh (Universiti Sains Malaysia)

저널정보: 대한백신학회 Clinical and Experimental Vaccine Research Clinical and Experimental Vaccine Research Vol.9 No.1

발행연도: 2020.1

수록면: 15 - 25 (11page)

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Purpose: In the developing world, bacillary dysentery is one of the most common communicable diarrheal infections. There are approximately 169 million cases of shigellosis reported worldwide. The disease is transmitted by a group of Gram-negative intracellular enterobacteria known as Shigella flexneri, S. sonnei, S. dysenteriae, and S. boydii. Conventional treatment regimens for Shigella have been less effective due to the development of resistant strains against antibiotics. Therefore, an effective vaccine for the long term control of Shigella transmission is urgently needed. Materials and Methods: In this study, a reverse vaccinology approach was employed to identify most conserved and immunogenic outer membrane proteins (OMPs) of S. flexneri 2a. Results: Five OMPs including fepA, ompC, nlpD_1, tolC, and nlpD_2 were identified as potential vaccine candidates. Protein-protein interactions analysis using STRING software (https://string-db.org/) revealed that five of these OMPs may potentially interact with other intracellular proteins which are involved in beta-lactam resistance pathway. B- and T-cell epitopes of the selected OMPs were predicted using BCPred as well as Propred I and Propred (http://crdd.osdd.net/raghava/propred/), respectively. Each of these OMPs contains regions which are capable to induce B- and T-cell immune responses. Conclusion: Analysis acquired from this study showed that five selected OMPs have great potential for vaccine development against S. flexneri infection. The predicted immunogenic epitopes can also be used for development of peptide vaccines or multi-epitope vaccines against human shigellosis. Reverse vaccinology is a promising strategy for the discovery of potential vaccine candidates which can be used for future vaccine development against global persistent infections.

#Antigens #Bioinformatics #Bacterial outer membrane protein #Shigella flexneri #Vaccines

참고문헌 (42)

참고문헌 신청

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Killackey SA / 2016 / Cellular aspects of Shigella pathogenesis : focus on the manipulation of host cell processes / Front Cell Infect Microbiol 6:38

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Banga Singh KK / 2011 / A 9-year study of shigellosis in Northeast Malaysia : antimicrobial susceptibility and shifting species dominance / Z Gesundh Wiss 19:231~236

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