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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 51 - 60 (10page)
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Few studies have evaluated the apoptosis-inducing efficacy of NaF on cancer cells in vitro but there has been no previous investigation of the apoptotic effects of NaF on human oral squamous cell carcinoma cells. In this study, we have investigated the mechanisms underlying the apoptotic response to NaF treatment in the YD9 human squamous cell carcinoma cell line. The viability of YD9 cells and their growth inhibition were assessed by MTT and clonogenic assays, respectively.
Hoechst staining, DNA electrophoresis and TUNEL staining were conducted to detect apoptosis. YD9 cells were treated with NaF, and western blotting, immunocytochemistry,confocal microscopy, FACScan flow cytometry, and MMP and proteasome activity assays were performed sequentially.
The NaF treatment resulted in a time- and dose-dependent decrease in YD9 cell viability, a dose-dependent inhibition of cell growth, and the induction of apoptotic cell death. The apoptotic response of these cells was manifested by nuclear condensation, DNA fragmentation, the reduction of MMP and proteasome activity, a decreased DNA content, the release of cytochrome c into the cytosol, the translocation of AIF and DFF40 (CAD) into the nucleus, a significant shift of the Bax/Bcl-2 ratio, and the activation of caspase-9, caspase-3, PARP, Lamin A/C and DFF45 (ICAD). Furthermore, NaF treatment resulted in the downregulation of G1 cell cyclerelated proteins, and upregulation of p53 and the Cdk inhibitor p27KIP1. Taken collectively, our present findings demonstrate that NaF strongly inhibits YD9 cell proliferation by modulating the expression of G1 cell cycle-related proteins and inducing apoptosis via mitochondrial and caspase pathways.
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참고문헌 (27)
참고문헌 신청
[학술지(정기간행물)] -
Acehan D / 2002 / Three-dimensional structure of the apoptosome: Implications for assembly, procaspase-9 binding, and activation / Mol Cell 9 : 423 ~ 432
Anuradha CD / 2000 / Fluoride induces apoptosis by caspase-3 activation in human leukemia HL-60 cells / Arch Toxicol 74 : 226 ~ 230
Anuradha CD / 2001 / Oxidative damage to mitochondria is a preliminary step to caspase-3 activation in fluoride-induced apoptosis in HL-60 cells / Free Radic Biol Med 31 : 36
Barczyk K / 2005 / Serum cytochrome c indicates in vivo apoptosis and can serve as a prognostic marker during cancer therapy / Int J Cancer 116 : 167 ~ 173
Acehan D / 2002 / Three-dimensional structure of the apoptosome: Implications for assembly, procaspase-9 binding, and activation / Mol Cell 9 : 423 ~ 432
Anuradha CD / 2000 / Fluoride induces apoptosis by caspase-3 activation in human leukemia HL-60 cells / Arch Toxicol 74 : 226 ~ 230
Anuradha CD / 2001 / Oxidative damage to mitochondria is a preliminary step to caspase-3 activation in fluoride-induced apoptosis in HL-60 cells / Free Radic Biol Med 31 : 36
Barczyk K / 2005 / Serum cytochrome c indicates in vivo apoptosis and can serve as a prognostic marker during cancer therapy / Int J Cancer 116 : 167 ~ 173
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