지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 708 - 716 (9page)
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초록· 키워드
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Purpose: Oral squamous carcinoma (OSCC) cells exhibit resistance to chemotherapeutic agent-mediated apoptosis in the late stage of malignancy. Increased levels of heat shock proteins 70 (HSP70) in cancer cells are known to confer resistance to apoptosis. Since recent advances in the understanding of bacterial toxins have produced new strategies for the treatment of cancers, we investigated the effect of Pseudomonas aeruginosa exotoxin A (PEA) on HSP70 expression and induction of apoptosis in chemoresistant OSCC cell line (YD-9). Materials and Methods:The apoptotic effect of PEA on chemoresistant YD-9 cells was confirmed by MTT,Hoechst and TUNEL stains, DNA electrophoresis, and Western blot analysis.
Results: While YD-9 cells showed high resistance to chemotherapeutic agents such as etoposide and 5-fluorouraci (5-FU), HSP70 antisense oligonucelotides sensitized chemoresistant YD-9 cells to etoposide and 5-FU. On the other hand, PEA significantly decreased the viability of YD-9 cells by deteriorating the HSP70-relating protecting system through inhibition of HSP70 expression and inducing apoptosis in YD-9 cells. Apoptotic manifestations were evidenced by changes in nuclear morphology, generation of DNA fragmentation, and activation of caspases. While p53, p21, and E2F-1 were upregulated, cdk2 and cyclin B were downregulated by PEA treatment, suggesting that PEA caused cell cycle arrest at the G2/M checkpoint.
Conclusion: Therefore, these results indicate that PEA reduced the chemoresistance through inhibition of HSP70 expression and also induced apoptosis in chemoresistant YD-9 cells.
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참고문헌 (23)
참고문헌 신청
Alley SC / 2009 / The pharmacologic basis for antibody-auristatin conjugate activity / J Pharmacol Exp Ther 330 : 932 ~ 938
Andersson Y / 2004 / Downregulation of the antiapoptotic MCL-1 protein and apoptosis in MA-11 breast cancer cells induced by an anti-epidermal growth factor receptor-Pseudom
Beere HM / 2001 / Stress management - heat shock protein-70 and the regulation of apoptosis / Trends Cell Biol 11 : 6 ~ 10
Bickels J / 2002 / Coley’s toxin:historical perspective / Isr Med Assoc J 4 : 471 ~ 472
Cho SJ / 2003 / Induction of apoptosis and expression of apoptosis related genes in human epithelial carcinoma cells by Helicobacter pylori VacA toxin / Toxicon 42 : 601 ~ 6
Andersson Y / 2004 / Downregulation of the antiapoptotic MCL-1 protein and apoptosis in MA-11 breast cancer cells induced by an anti-epidermal growth factor receptor-Pseudom
Beere HM / 2001 / Stress management - heat shock protein-70 and the regulation of apoptosis / Trends Cell Biol 11 : 6 ~ 10
Bickels J / 2002 / Coley’s toxin:historical perspective / Isr Med Assoc J 4 : 471 ~ 472
Cho SJ / 2003 / Induction of apoptosis and expression of apoptosis related genes in human epithelial carcinoma cells by Helicobacter pylori VacA toxin / Toxicon 42 : 601 ~ 6
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아밀로이드 베타에 의한 신경세포 사멸과 이에 대한 열충격 단백질 90의 세포 보호효과
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