지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 155 - 162 (8page)
이용수
초록· 키워드
오류제보하기
Eugenol (4-allyl-2-methoxyphenol) is a naturally occurring phenolic compound that is widely used in dentistry as a component of zinc oxide eugenol cement that is commonly applied to the mouth environment. Cisplatin is one of the most potent known anticancer agents and shows significant clinical activity against a variety of solid tumors. This study was undertaken to investigate the synergistic apoptotic effects of co-treatments with eugenol and cisplatin on human melanoma (G361) cells.
To investigate whether this co-treatment efficiently reduces the viability of G361 cells compared with each single treatment, an MTT assay was conducted. The induction and augmentation of apoptosis were confirmed by DNA electrophoresis, Hoechst staining and an analysis of DNA hypoploidy. Western blot analysis and immunofluorescent staining were also performed to evaluate the expression levels and the translocation of apoptosis-related proteins following this co-treatment. Furthermore,proteasome activity and mitochondrial membrane potential (MMP) changes were also assayed. The results indicated that a co-treatment with eugenol and cisplatin induced multiple pathways and processes associated with an apoptotic response in G361 cells including nuclear condensation,DNA fragmentation, a reduction in MMP and proteasome activity, the increase and decrease of Bax and Bcl-2, a decreased DNA content, the release of cytochrome c into the cytosol, the translocation of AIF and DFF40 (CAD)into the nucleus, and the activation of caspase-9, caspase-7,caspase-3, PARP and DFF45 (ICAD). In contrast, separate treatments of 300 μM eugenol or 3 μM cisplatin for 24 h did not induce apoptosis. Our present data thus suggest that a combination therapy of eugenol and cisplatin is a potential treatment strategy for human melanoma.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
/ 1985 / IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, in Allyl Compounds, Aldehydes, Epoxides and Peroxides : 75 ~
Acehan D / 2002 / Three-dimensional structure of the apoptosome: Implications for assembly, procaspase-9 binding, and activation / Mol Cell 9 : 423 ~ 432
Adhami VM / 2007 / Combined inhibitory effects of green tea polyphenols and selective cyclooxygenase-2 inhibitors on the growth of human prostate cancer cells both invitro an
Cheng AC / 2007 / Induction of apoptosis by Uncaria tomentosa through reactive oxygen species production, cytochrome c release, and caspases activation in human leukemia cell
Cooley ME / 1994 / Cisplatin: a clinical review. Part I--Current uses of cisplatin and administration guidelines / Cancer Nurs 17 : 173 ~ 184
Acehan D / 2002 / Three-dimensional structure of the apoptosome: Implications for assembly, procaspase-9 binding, and activation / Mol Cell 9 : 423 ~ 432
Adhami VM / 2007 / Combined inhibitory effects of green tea polyphenols and selective cyclooxygenase-2 inhibitors on the growth of human prostate cancer cells both invitro an
Cheng AC / 2007 / Induction of apoptosis by Uncaria tomentosa through reactive oxygen species production, cytochrome c release, and caspases activation in human leukemia cell
Cooley ME / 1994 / Cisplatin: a clinical review. Part I--Current uses of cisplatin and administration guidelines / Cancer Nurs 17 : 173 ~ 184
이 논문의 저자 정보
최근 본 자료
댓글(0)
0