지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 834 - 841 (8page)
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Purpose: To investigate the anti-tumor effect of capsaicin on human pharyngeal squamous carcinoma cells (FaDu). Materials and Methods: The expression of apoptosis/cell cycle-related proteins (or genes) was examined by reverse transcriptase-polymerase chain reaction, western blotting and ELISA methods, while the apoptotic cell population, cell morphology and DNA fragmentation levels were assessed using flow cytometry, fluorescence microscopy and agarose gel electrophoresis.
Results: Capsaicin was found to inhibit the growth and proliferation of FaDu cells in a dose- and time-dependent manner. Apoptotic cell death was confirmed by observing increases in nuclear condensation, nuclear DNA fragmentation and sub-G1 DNA content. The observed increase in cytosolic cytochrome c, activation of caspase 3 and PARP (p85) levels following capsaicin treatment indicated that the apoptotic response was mitochondrial pathway-dependent. Gene/protein expression analysis of Bcl-2, Bad and Bax further revealed decreased anti-apoptotic Bcl-2 protein and increased pro-apoptotic Bad/Bax expression. Furthermore, capsaicin suppressed the cell cycle progression at the G1/S phase in FaDu cells by decreasing the expression of the regulators of cyclin B1 and D1, as well as cyclin-dependent protein kinases cdk-1, cdk-2 and cdk-4. Conclusion: Our current data show that capsaicin induces apoptosis in FaDu cells and this response is associated with mitochondrial pathways, possibly by mediating cell cycle arrest at G1/S.
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참고문헌 (25)
참고문헌 신청
Amantini C / 2007 / Capsaicin-induced apoptosis of glioma cells is mediated by TRPV1 vanilloid receptor and requires p38 MAPK activation / J Neurochem 102 : 977 ~ 990
Borner C / 2003 / The Bcl-2 protein family: sensors and checkpoints for life-or-death decisions / Mol Immunol 39 : 615 ~ 647
Chen AY / 2000 / Cancer of the oral cavity / Curr Probl Surg 37 : 633 ~ 731
Cho YS / 2003 / Enhanced cough response to hyperpnea with cold air challenge in chronic cough patients showing increased cough sensitivity to inhaled capsaicin / Allergy 58
Chu-Sook Kim / 2004 / Capsaicin, a Spicy Component of Hot Pepper, Induces Apoptosis by Activation of the Peroxisome Proliferator-Activated receptor gamma in HT-29 human colo
Borner C / 2003 / The Bcl-2 protein family: sensors and checkpoints for life-or-death decisions / Mol Immunol 39 : 615 ~ 647
Chen AY / 2000 / Cancer of the oral cavity / Curr Probl Surg 37 : 633 ~ 731
Cho YS / 2003 / Enhanced cough response to hyperpnea with cold air challenge in chronic cough patients showing increased cough sensitivity to inhaled capsaicin / Allergy 58
Chu-Sook Kim / 2004 / Capsaicin, a Spicy Component of Hot Pepper, Induces Apoptosis by Activation of the Peroxisome Proliferator-Activated receptor gamma in HT-29 human colo
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