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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 159 - 167 (9page)
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Curcumin is a widely used flavoring agent in food, and it hasbeen reported to inhibit cell growth, to induce apoptosis, andto have antitumor activity in many cancers. Cisplatin is one ofthe most potent known anticancer agents and showssignificant clinical activity against a variety of solid tumors.
This study was undertaken to investigate the synergisticapoptotic effects of co-treatment with curcumin and cisplatinon human tongue SCC25 cells. To investigate whether theco-treatment efficiently reduced the viability of the SCC25cells compared with the two treatments separately, an MTTassay was conducted. The induction and the augmentation ofapoptosis were confirmed by DNA electrophoresis, Hoechststaining, and an analysis of DNA hypoploidy. Western blot,MMP and immunofluorescence tests were also performed toevaluate the expression levels and the translocation ofapoptosis-related proteins following the co-treatment.
In this study, following the co-treatment with curcumin andcisplatin, the SCC25 cells showed several forms of apoptoticmanifestation, such as nuclear condensation, DNAfragmentation, reduction of MMP, increased levels of Bax,decreased levels of Bcl-2, and decreased DNA content. Inaddition, they showed a release of cytochrome c into thecytosol, translocation of AIF and DFF40 (CAD) to the nuclei,and activation of caspase-7, caspase-3, PARP, and DFF45(ICAD). In contrast, separate treatments of 5 μM of curcuminor 4 μg/ml of cisplatin, for 24 hours, did not induce apoptosis.
Therefore, our data suggest that combination therapy withcurcumin and cisplatin could be considered as a noveltherapeutic strategy for human oral squamous cell carcinoma.
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참고문헌 (46)
참고문헌 신청
Gonzalez VM / 2001 / Is cisplatin-induced cell death always produced by apoptosis? / Mol Pharmacol 59:657~663
Seki K / 2000 / Cisplatin(CDDP)specifically induces apoptosis via sequential activation of caspase-8, -3 and-6 in osteosarcoma / Cancer Chemother Pharmacol 45:199~206
Zhou H / 2004 / The induction of cell cycle regulatory and DNA repair proteins in cisplatin-induced acute renal failure / Toxicol Appl Pharmacol 200:111~120
Fox SA / 2005 / Cisplatin and TNF-alpha downregulate transcription of Bcl-xL in murine malignant mesothelioma cells / Biochem Biophys Res Commun 337:983~991
Garcia-Berrocal JR / 2007 / The anticancer drug cisplatin induces an intrinsic apoptotic pathway inside the inner ear / Br J Pharmacol 152:1012~1020
Seki K / 2000 / Cisplatin(CDDP)specifically induces apoptosis via sequential activation of caspase-8, -3 and-6 in osteosarcoma / Cancer Chemother Pharmacol 45:199~206
Zhou H / 2004 / The induction of cell cycle regulatory and DNA repair proteins in cisplatin-induced acute renal failure / Toxicol Appl Pharmacol 200:111~120
Fox SA / 2005 / Cisplatin and TNF-alpha downregulate transcription of Bcl-xL in murine malignant mesothelioma cells / Biochem Biophys Res Commun 337:983~991
Garcia-Berrocal JR / 2007 / The anticancer drug cisplatin induces an intrinsic apoptotic pathway inside the inner ear / Br J Pharmacol 152:1012~1020
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