지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.12
- 수록면
- 144 - 152 (9page)
- DOI
- http://dx.doi.org/10.13041/jpvm.2015.39.4.144
이용수
초록· 키워드
오류제보하기
Brucella abortus is a well-known intracellular zoonotic pathogen. Despite significant research to understand the pathogenesis, underlying mechanisms of the bacterial infection are not yet understood. Thus, prevention and control of the B. abortus infection is problematic in animal and human.
Therefore, several methods involving random mutation have been used to identify the mechanisms and provide a solution for control and prevention of Brucella infection. B. abortus mutants were generated by random insertion of a transposon, Ez-Tn5TM pMODTM-3 <R6Kγori/MCS> into a chromosome. Characteristics of these mutants were investigated using biochemical testing, growth features, determination of biovar, antibiotic resistance and detection of virulence factors, T4SS, PGK, and CGS. In biochemical testing, B. abortus mutants were categorized according to 7 groups with different condition of ILATk, SUTC, and ELLM. Different growth features were also observed between wild type and mutants. In addition, both B. abortus wild type and mutants were determined as biovar type 1 by biovar test. Three virulence factors, T4SS, PGK, and CGS were detected by PCR. Therefore, B. abortus mutants were characterized by analysis of phenotyping and it might be useful for further studies of known pathogenesis of B. abortus infection and for identification of diagnostic markers of brucellosis.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Boschiroli ML / 2001 / Brucellosis:a worldwide zoonosis / Curr Opin Microbiol 4:58~64
Cha SB / 2010 / Comparative study of gamma interferon production in mice immunized with outer membrane proteins and whole bacteria of Brucella abortus / Jpn J Infect Dis 63:49~51
Cha SB / 2012 / Generation and envelope protein analysis of internalization defective Brucella abortus mutants in professional phagocytes, RAW 264.7 / FEMS Immunol Med Microbiol 64:244~254
de Souza Filho JA / 2015 / Mutant Brucella abortus membrane fusogenic protein induces protection against challenge infection in mice / Infect Immun 83:1458~1464
Delrue RM / 2001 / Identification of Brucella spp. genes involved in intracellular trafficking / Cell Microbiol 3:487~497
Cha SB / 2010 / Comparative study of gamma interferon production in mice immunized with outer membrane proteins and whole bacteria of Brucella abortus / Jpn J Infect Dis 63:49~51
Cha SB / 2012 / Generation and envelope protein analysis of internalization defective Brucella abortus mutants in professional phagocytes, RAW 264.7 / FEMS Immunol Med Microbiol 64:244~254
de Souza Filho JA / 2015 / Mutant Brucella abortus membrane fusogenic protein induces protection against challenge infection in mice / Infect Immun 83:1458~1464
Delrue RM / 2001 / Identification of Brucella spp. genes involved in intracellular trafficking / Cell Microbiol 3:487~497
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Analysis of protein expression in mutants with different growth rates by two-dimensional gel electrophoresis and LC-MS/MS peptide analysis
Journal of Veterinary Science
2018 .03
-
Different invasion efficiencies of Brucella abortus wild-type and mutants in RAW 264.7 and THP-1 phagocytic cells and HeLa non-phagocytic cells
Korean Journal of Veterinary Research(대한수의학회지)
2018 .07
-
Isolation and characterization of Brucella abortus isolates from wildlife species in South Korea
Korean Journal of Veterinary Research(대한수의학회지)
2016 .10
-
Protection of palmitic acid treatment in RAW264.7 cells and BALB/c mice during Brucella abortus 544 infection
Journal of Veterinary Science
2021 .03
-
A Novel PCR Assay for Detecting Brucella abortus and Brucella melitensis
Osong Public Health and Research Persptectives
2017 .01
-
Inhibitory effect of red ginseng acidic polysaccharide from Korean red ginseng on phagocytic activity and intracellular replication of Brucella abortus in RAW 264.7 cells
Journal of Veterinary Science
2016 .09
-
The in vitro effects of synthetic complement peptide C3a during Brucella abortus 544 infection in a murine professional phagocyte RAW264.7 cell line
예방수의학회지
2021 .12
-
Succinic acid inhibits the uptake of Brucella abortus 544 into RAW 264.7 cells and promotes survival of B. abortus in ICR mice
예방수의학회지
2021 .12
-
Vaccination of 50S ribosomal protein L27 decreased susceptibility to Brucella abortus 544 infection in BALB/c mice
예방수의학회지
2019 .01
-
Immunization with Brucella abortus recombinant proteins protects BALB/c mice from Brucella abortus 544 infection
예방수의학회지
2018 .12
-
Immunization of a combination of four recombinant Brucella abortus proteins Adk, Ndk, L7/L12 and SecB induced protective immune responses in BALB/c mice against a virulent strain B. abortus 544 infection
예방수의학회지
2020 .01
-
Vaccination of goats with a combination Salmonella vector expressing four Brucella antigens (BLS, PrpA, Omp19, and SOD) confers protection against Brucella abortus infection
Journal of Veterinary Science
2018 .09
-
Resistance to Brucella abortus 544 challenge in BALB/c mice by inoculation of highly immunogenic multiple B. abortus antigens
예방수의학회지
2019 .01
-
Nucleomodulin BspJ as an effector promotes the colonization of Brucella abortus in the host
Journal of Veterinary Science
2022 .01
-
Protective effects of recombinant Brucella abortus protein Fe/Mn superoxide dismutase against a virulent strain B. abortus 544 infection in BALB/c mice
예방수의학회지
2022 .12
-
Tannic acid-mediated immune activation attenuates infection in mice
Journal of Veterinary Science
2018 .01
-
First Case of Human Brucellosis Caused by Brucella melitensis in Korea
Annals of Laboratory Medicine
2016 .01
-
Evaluation of recombinant AspC of Brucella abortus for serological diagnosis of bovine brucellosis in Korea
예방수의학회지
2015 .06
-
Development of a Method for Manufacturing External Quality Assessment Material for Genetic Testing of Solid Tumors Using Mutant and Wild-Type Cell Lines
Journal of Laboratory Medicine And Quality Assurance
2021 .01
-
Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein
Journal of Veterinary Science
2016 .03
이 논문의 저자 정보
최근 본 자료
댓글(0)
0