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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.6
- 수록면
- 426 - 433 (8page)
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Ephrin-A5 has been implicated in the regulation of brain morphogenesis and axon pathfinding. In this study, we used bacterial homologous recombination to express a LacZ reporter in various ephrin-A5 BAC clones to identify elements that regulate ephrin-A5 gene expression during mesencephalon development. We found that there is mesencephalon-specific enhancer activity localized to a specific +25.0 kb to +30.5 kb genomic region in the first intron of ephrin-A5. Further comparative genomic analysis indicated that two evolutionary conserved regions, ECR1 and ECR2, were present within this 5.5 kb region. Deletion of ECR1 from the enhancer resulted in disrupted mesencephalon-specific enhancer activity in transgenic embryos. We also found a consensus binding site for basic helix-loop-helix (bHLH) transcription factors (TFs) in a highly conserved region at the 3?-end of ECR1. We further demonstrated that specific deletion of the bHLH TF binding site abrogated the mesencephalon-specific enhancer activity in transgenic embryos. Finally, both electrophoretic mobility shift assay and luciferase-based transactivation assay revealed that the transcription factor Ascl1 bound the bHLH consensus binding site in the mesencephalon-specific ephrin-A5 enhancer in vitro. Together, these results suggest that the bHLH TF binding site in ECR1 is involved in the positive regulation of ephrin-A5 gene expression during the development of the mesencephalon.
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참고문헌 (25)
참고문헌 신청
Carreres, M. I. / 2011 / Transcription factor Foxd1 is required for the specification of the temporal retina in mammals / J. Neurosci 31:5673~5681
Casarosa, S. / 1999 / Mash1 regulates neurogenesis in the ventral telencephalon / Development 126:525~534
Castro, D. S. / 2006 / Proneural bHLH and Brn proteins coregulate a neurogenic program through cooperative binding to a conserved DNA motif / Dev. Cell 11:831~844
Castro, D. S. / 2011 / A novel function of the proneural factor Ascl1 in progenitor proliferation identified by genome-wide characterization of its targets / Genes Dev 25:930~945
Coulthard, M. G. / 2002 / The role of the Eph-ephrin signalling system in the regulation of developmental patterning / Int. J. Dev. Biol 46:375~384
Casarosa, S. / 1999 / Mash1 regulates neurogenesis in the ventral telencephalon / Development 126:525~534
Castro, D. S. / 2006 / Proneural bHLH and Brn proteins coregulate a neurogenic program through cooperative binding to a conserved DNA motif / Dev. Cell 11:831~844
Castro, D. S. / 2011 / A novel function of the proneural factor Ascl1 in progenitor proliferation identified by genome-wide characterization of its targets / Genes Dev 25:930~945
Coulthard, M. G. / 2002 / The role of the Eph-ephrin signalling system in the regulation of developmental patterning / Int. J. Dev. Biol 46:375~384
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