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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 763 - 772 (10page)
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초록· 키워드
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Periodontitis is characterized by the loss of periodontal tissues, especially alveolar bone. Common therapies cannot satisfactorily recover lost alveolar bone. Periodontal ligament stem cells (PDLSCs) possess the capacity of self-renewal and multilineage differentiation and are likely to recover lost alveolar bone. In addition, periodontitis is accompanied by hypoxia, and hypoxia-inducible factor-1α (HIF-1α) is a master transcription factor in the response to hypoxia. Thus, we aimed to ascertain how hypoxia affects runt-related transcription factor 2 (RUNX2), a key osteogenic marker, in the osteogenesis of PDLSCs. In this study, we found that hypoxia enhanced the protein expression of HIF-1α, vascular endothelial growth factor (VEGF), and RUNX2 ex vivo and in situ. VEGF is a target gene of HIF-1α, and the increased expression of VEGF and RUNX2 proteins was enhanced by cobalt chloride (CoCl2, 100 μmol/L), an agonist of HIF-1α, and suppressed by 3-(5′-hydroxymethyl-2′-furyl)-1-benzyl indazole (YC-1, 10 μmol/L), an antagonist of HIF-1α. In addition, VEGF could regulate the expression of RUNX2, as RUNX2 expression was enhanced by human VEGF (hVEGF165) and suppressed by VEGF siRNA. In addition, knocking down VEGF could decrease the expression of osteogenesis-related genes, i.e., RUNX2, alkaline phosphatase (ALP), and type I collagen (COL1), and hypoxia could enhance the expression of ALP, COL1, and osteocalcin (OCN) in the early stage of osteogenesis of PDLSCs. Taken together, our results showed that hypoxia could mediate the expression of RUNX2 in PDLSCs via HIF-1α-induced VEGF and play a positive role in the early stage of osteogenesis of PDLSCs
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참고문헌 (3)
참고문헌 신청
Abbayya, K. / 2015 / Cell- and gene-based therapeutics for periodontal regeneration / Int. J. Prev. Med 6:110
Campbell, L. / 2016 / T cells, teeth and tissue destruction - what do T cells do in periodontal disease? / Mol. Oral Microbiol 31:445~456
Chen, D. / 2017 / Comparison of HIF1AAS1 and HIF1AAS2 in regulating HIF1alpha and the osteogenic differentiation of PDLCs under hypoxia / Int. J. Mol. Med 40:1529~1536
Campbell, L. / 2016 / T cells, teeth and tissue destruction - what do T cells do in periodontal disease? / Mol. Oral Microbiol 31:445~456
Chen, D. / 2017 / Comparison of HIF1AAS1 and HIF1AAS2 in regulating HIF1alpha and the osteogenic differentiation of PDLCs under hypoxia / Int. J. Mol. Med 40:1529~1536
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