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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 370 - 376 (7page)
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초록· 키워드
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To develop novel antibiotic peptides useful as therapeutic drugs, the enantiomeric analogue of pleurocidin (Ple), which is a well known 25-mer antimicrobial peptide, was designed for proteolytic resistance by D-amino acids substitution. The proteolytic resistance was confirmed by using HPLC after the digestion with various proteases. To investigate the antibiotic effect of L- and D-Ple, the antibacterial activity and hemolytic effect were tested against human erythrocytes. The D-Ple showed a decreased antibacterial activity and a dramatically decreased hemolytic activity compared with L-Ple. The hemolytic effect of analogue was further confirmed by using calcein leakage measurement with liposome. To elucidate these results, the secondary structure of the peptides was investigated by using circular dichroism spectroscopy. The results revealed that D-Ple, as well as L-Ple, had typical α-helical structures which were mirror images, with a different helicity. These results suggested that the discrepancy of the structure between the two peptides made their antibacterial activity distinct.
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참고문헌 (23)
참고문헌 신청
/ 2005 / Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing, fifteenth informational supplement, approved standard MS100-S15, CLS
Bessalle R / 1990 / All-D-magainin: chirality, antimicrobial activity and proteolytic resistance / FEBS Lett 274 : 151 ~ 5
Blondelle SE / 1992 / Design of model amphipathic peptides having potent antimicrobial activities / Biochemistry 31 : 12688 ~ 94
Cole AM / 1997 / Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder / J Biol Chem 272 : 12008 ~ 13
DeGrado WF / 1981 / Design, synthesis and characterization of a cytotoxic peptide with melittin-like activity / J Am Chem Soc 103 : 679 ~ 81
Bessalle R / 1990 / All-D-magainin: chirality, antimicrobial activity and proteolytic resistance / FEBS Lett 274 : 151 ~ 5
Blondelle SE / 1992 / Design of model amphipathic peptides having potent antimicrobial activities / Biochemistry 31 : 12688 ~ 94
Cole AM / 1997 / Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder / J Biol Chem 272 : 12008 ~ 13
DeGrado WF / 1981 / Design, synthesis and characterization of a cytotoxic peptide with melittin-like activity / J Am Chem Soc 103 : 679 ~ 81
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