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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제10호
- 발행연도
- 2013.1
- 수록면
- 1,386 - 1,394 (9page)
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In our previous study, the 25-mer antimicrobial peptide pleurocidin (Ple) had been thought to induce apoptosis in Candida albicans. This study demonstrated that reactive oxygen species (ROS) production was a major cause of Ple-induced apoptosis. Four truncated analogs were synthesized to understand the functional roles in the N- and C-terminal regions of Ple on the apoptosis. Ple, Ple (4-25), Ple (1-22), and Ple (1-19) produced ROS, including hydroxyl radicals,on the order of [Ple > Ple (1-22) > Ple (4-25) > Ple (1-19)], whereas Ple (7-25) did not induce any ROS production. The results suggested that the N-terminal deletion affected the ROS-inducing activities much more than that of the C-terminal deletion, and net hydrophobicity [Ple > Ple (1-22) > Ple (4-25) > Ple (1-19) > Ple (7-25)] was related to ROS generation rather than other primary factors like net charge. Hence, we focused on the N-terminal-truncated peptides, Ple (4-25) and Ple (7-25), and examined other apoptotic features, including mitochondrial membrane depolarization, caspase activation, phosphatidylserine externalization, and DNA and nuclear fragmentation. The results also confirmed the disappearance of apoptotic activity of Ple (7-25) by the truncation of the N-terminal region (1-6) and the specific activity patterns between Ple and analogs. In conclusion, the N-terminal region of Ple played an important role in apoptosis.
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참고문헌 (25)
참고문헌 신청
Aerts AM / 2009 / The antifungal plant defensin RsAFP2 from radish induces apoptosis in a metacaspase independent way in Candida albicans / FEBS Lett 583 : 2513 ~ 2516
Bossy-Wetzel E / 1998 / Mitochondrial cytochrome c release in apoptosis occurs upstream of DEVDspecific caspase activation and independently of mitochondrial transmembrane de
Brogden KA. / 2005 / Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? / Nat. Rev. Microbiol 3 : 238 ~ 250
Cho J / 2011 / Oxidative stress by antimicrobial peptide pleurocidin triggers apoptosis in Candida albicans / Biochimie 93 : 1873 ~ 1879
Cole AM / 1997 / Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder / J. Biol. Chem 272 : 12008 ~ 12013
Bossy-Wetzel E / 1998 / Mitochondrial cytochrome c release in apoptosis occurs upstream of DEVDspecific caspase activation and independently of mitochondrial transmembrane de
Brogden KA. / 2005 / Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? / Nat. Rev. Microbiol 3 : 238 ~ 250
Cho J / 2011 / Oxidative stress by antimicrobial peptide pleurocidin triggers apoptosis in Candida albicans / Biochimie 93 : 1873 ~ 1879
Cole AM / 1997 / Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder / J. Biol. Chem 272 : 12008 ~ 12013
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