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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제10호
- 발행연도
- 2012.1
- 수록면
- 1,367 - 1,374 (8page)
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초록· 키워드
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Pleurocidin, a 25-mer antimicrobial peptide, has been known to exhibit potent antibacterial activity. To investigate the functional roles in N- and C-terminal regions of pleurocidin on the antibacterial activity, we designed four truncated analogs. The antibacterial susceptibility testing showed that pleurocidin and its analogs exerted antibacterial effect against various bacterial strains and further possessed specific activity patterns corresponding with their hydrophobic scale [pleurocidin > Anal 3 (1-22) >Anal 1 (4-25) > Anal 4 (1-19) > Anal 2 (7-25)]. Fluorescence experiments using 1,6-diphenyl-1,3,5-hexatriene (DPH)and 3,3'-dipropylthiadicarbocyanine iodide [diSC3(5)]indicated that the differences in antibacterial activity of the peptides were caused by its membrane-active mechanisms including membrane disruption and depolarization. Blue shift in tryptophan fluorescence demonstrated that the decrease in net hydrophobicity attenuates the binding affinity of pleurocidin to interact with plasma membrane.
Therefore, the present study suggests that hydrophobicity in the N- and C-terminal regions of pleurocidin plays a key role in its antibacterial activity.
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참고문헌 (22)
참고문헌 신청
CLSI. / 2005 / Clinical and Laboratory Standards Institute Performance Standards for Antimicrobial Susceptibility Testing, Fifteenth Informational Supplement, Approved Standard MS100-
Christiaens, B. / 2002 / Tryptophan fluorescence study of the interaction of penetration peptides with model membranes / Eur. J. Biochem 269 : 2918 ~ 2926
Cole, A. M. / 1997 / Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder / J. Biol. Chem 272 : 12008 ~ 12013
Dupuy, B. / 1997 / Spectral properties of a fluorescent probe, all-trans-1,6-diphenyl-1,3,5-hexatriene. Solvent and temperature effects / Analyst 122 : 783 ~ 786
Epand, R. F. / 2008 / Dual mechanism of bacterial lethality for a cationic sequence-random copolymer that mimics host-defense antimicrobial peptides / J. Mol. Biol 379 : 38 ~
Christiaens, B. / 2002 / Tryptophan fluorescence study of the interaction of penetration peptides with model membranes / Eur. J. Biochem 269 : 2918 ~ 2926
Cole, A. M. / 1997 / Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder / J. Biol. Chem 272 : 12008 ~ 12013
Dupuy, B. / 1997 / Spectral properties of a fluorescent probe, all-trans-1,6-diphenyl-1,3,5-hexatriene. Solvent and temperature effects / Analyst 122 : 783 ~ 786
Epand, R. F. / 2008 / Dual mechanism of bacterial lethality for a cationic sequence-random copolymer that mimics host-defense antimicrobial peptides / J. Mol. Biol 379 : 38 ~
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