지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 448 - 454 (7page)
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초록· 키워드
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Background : Plasmid-mediated AmpC β-lactamases (PABLs) have been detected in the strains of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. PABLs may be difficult to detect and might interfere in the therapeutic and infection-control processes. Although several PABL-detection methods based on phenotypes have been reported, the Clinical and Laboratory Standards Institute currently does not recommend a routine detection method for PABLs. The aim of this study is to compare the performances of 3 phenotypic PABL detection methods.
Methods : Total 276 non-duplicated clinical isolates of E. coli (N=97), K. pneumoniae (N=136), and P. mirabilis (N=43) were collected from 14 hospitals in Korea between April and June 2007 in a non-consecutive and non-random manner. Multiplex PCR was performed to detect the PABL genes. Further, 3 phenotypic detection methods-cephamycin-Hodge test, Tris-EDTA (TE) disk test, and combination-disk test with 3-aminophenylboronic acid (BA)-were performed using cefoxitin and cefotetan disks.
Results : PABL genes were detected by multiplex PCR in 122/276 isolates, including 14/97 E. coli, 105/136 K. pneumoniae, and 3/43 P. mirabilis isolates. The combination-disk test with BA showed higher sensitivity (98.4%), specificity (92.2%), and efficiency (96.3%) than the cephamycin-Hodge (76.2%, 96.1%, and 88.6%, respectively) and the TE-disk (80.3%, 91.6%, and 87.9%, respectively) tests.
Conclusions : The combination-disk test with BA is a simple, efficient, and interpretable test that can be applicable in clinical laboratories involved
in the detection of PABLs in clinical isolates of E. coli, K. pneumoniae, and P. mirabilis.
목차
등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Ardanuy C / 1998 / Outer membrane profiles of clonally related Klebsiella pneumoniae isolates from clinical samples and activities of cephalosporins and carbapenems / Antimic
Ayers LW / 1982 / Cefotetan, a new cephamycin: comparison of in vitro antimicrobial activity with other cephems, β-lactamase stability, and preliminary recommendations for
Beesley T / 1983 / The inhibition of class C β-lactamases by boronic acids / Biochem J 209 : 229 ~ 233
Black JA / 2005 / AmpC disk test for detection of plasmid-mediated AmpC β-lactamases in Enterobacteriaceae lacking chromosomal AmpC β-lactamases / J Clin Microbiol 43 : 311
Clinical and Laboratory Standards Institute / 2006 / Performance standards for antimicrobial susceptibility testing. Approved Standard, M02-A9. Wayne, PA: Clinical and Laboratory Stan
Ayers LW / 1982 / Cefotetan, a new cephamycin: comparison of in vitro antimicrobial activity with other cephems, β-lactamase stability, and preliminary recommendations for
Beesley T / 1983 / The inhibition of class C β-lactamases by boronic acids / Biochem J 209 : 229 ~ 233
Black JA / 2005 / AmpC disk test for detection of plasmid-mediated AmpC β-lactamases in Enterobacteriaceae lacking chromosomal AmpC β-lactamases / J Clin Microbiol 43 : 311
Clinical and Laboratory Standards Institute / 2006 / Performance standards for antimicrobial susceptibility testing. Approved Standard, M02-A9. Wayne, PA: Clinical and Laboratory Stan
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