지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 185 - 190 (6page)
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초록· 키워드
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Background : The aim of this study was to compare the BD Phoenix (Beckton Dickinson Diagnostic
Systems, USA) extended-spectrum β-lactamase (ESBL) test with the Clinical and Laboratory
Standards Institute (CLSI) ESBL phenotypic confirmatory test by disk diffusion (CLSI ESBL test) in
Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca and Proteus mirabilis.
Methods : We tested 224 clinical isolates of E. coli, K. pneumoniae, K. oxytoca and P. mirabilis
during May 2006 to March 2007. These isolates were examined by the Phoenix and the CLSI ESBL
tests simultaneously. For the isolates showing discordant results between the two tests, boronic
acid disk test was performed to differentiate AmpC β-lactamase and ESBL.
Results : Among the 224 clinical isolates, 75 and 79 isolates were positive for ESBL by CLSI ESBL
test and Phoenix test, respectively. Having detected 4 more isolates as ESBL-producers, Phoenix
test showed a 98.2% agreement with a 100% sensitivity and 97.3% specificity compared with CLSI
ESBL test. Among the four false positive isolates, three were AmpC-positive but ESBL-negative.
Conclusions : The BD Phoenix ESBL test was sensitive and specific, and can be used as a rapid
and reliable method to detect ESBL production in E. coli, Klebsiella species, and P. mirabilis. (Korean
J Lab Med 2008;28:185-90)
목차
등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Bradford PA / 2001 / Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat / Clin Microbiol
Bush K / 1995 / A functional classification scheme for beta-lactamases and its correlation with molecular structure / Antimicrob Agents Chemother 39 : 1211 ~ 1233
Clinical and Laboratory Standards Institute / 2007 / Performance standards for antimicrobial susceptibility testing / 17th informational supple ment
Coudron PE / 2005 / Inhibitor-based methods for detection of plasmidmediated AmpC beta-lactamases in Klebsiella spp., Escherichia coli, and Proteus mirabilis / J Clin Microb
Hong SG / 2001 / Molecular characteristics of extended-spectrum β-lactamases in clinical isolates of Enterobacteriaceae / Korean J Clin Pathol 21 : 495 ~ 504
Bush K / 1995 / A functional classification scheme for beta-lactamases and its correlation with molecular structure / Antimicrob Agents Chemother 39 : 1211 ~ 1233
Clinical and Laboratory Standards Institute / 2007 / Performance standards for antimicrobial susceptibility testing / 17th informational supple ment
Coudron PE / 2005 / Inhibitor-based methods for detection of plasmidmediated AmpC beta-lactamases in Klebsiella spp., Escherichia coli, and Proteus mirabilis / J Clin Microb
Hong SG / 2001 / Molecular characteristics of extended-spectrum β-lactamases in clinical isolates of Enterobacteriaceae / Korean J Clin Pathol 21 : 495 ~ 504
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