지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.8
- 수록면
- 857 - 868 (12page)
- DOI
- 10.1007/s13258-021-01098-9
이용수
초록· 키워드
오류제보하기
Background Hepatocellular carcinoma (HCC) is one of the most common human cancers. Long non-coding RNAs (lncRNAs) play pivotal roles in progression of various cancers, including HCC.
Objective We aimed to explore the exact role and underlying mechanism of lncRNA HOX transcript antisense intergenic RNA (HOTAIR) in HCC.
Methods Quantitative real time polymerase chain reaction (qRT-PCR) was carried out to determine the levels of HOTAIR, DEAH-box helicase 33 (DHX33) and miR-526b-3p. Western blot assay was used to detect the protein level of DHX33.
Besides, cell proliferation and apoptosis were assessed by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and fow cytometry analysis, respectively. Cell migration and invasion were detected by transwell assay. The interaction between miR-526b-3p and HOTAIR or DHX33 was predicted by starbase and confrmed by the dual-luciferase reporter assay. Murine xenograft model was established through injecting Huh7 cells transfected with sh-NC or sh-HOTAIR.
Results The levels of HOTAIR and DHX33 were increased in HCC tissues and cells. Knockdown of either HOTAIR or DHX33 suppressed proliferation, migration and invasion but increased apoptosis in HCC cells. Moreover, DHX33 overexpression reversed the suppressive efect of HOTAIR knockdown on progression of HCC cells. Interestingly, miR-526b-3p could directly bind to HOTAIR, and DHX33 was a direct target of miR-526b-3p. Additionally, interference of HOTAIR restrained the tumor growth by upregulating miR-526b-3p and downregulating DHX33 in vivo.
Conclusions HOTAIR knockdown suppressed cell proliferation, migration and invasion, and promoted apoptosis via regulating miR-526b-3p/DHX33 axis in HCC cells, providing a potential avenue for treatment of HCC.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Circulating HOTAIR LncRNA Is Potentially Up-regulated in Coronary Artery Disease
Genomics & informatics
2018 .01
-
Propofol enhanced the cell sensitivity to paclitaxel (PTX) in prostatic cancer (PC) through modulation of HOTAIR
Genes & Genomics
2021 .01
-
MiR-1299 functions as a tumor suppressor to inhibit the proliferation and metastasis of gastric cancer by targeting ARF6
Genes & Genomics
2022 .02
-
MicroRNA-766-3p Inhibits Tumour Progression by Targeting Wnt3a in Hepatocellular Carcinoma
Molecules and Cells
2018 .09
-
MicroRNA?9 inhibits proliferation and progression in retinoblastoma cells by targeting PTEN
Genes & Genomics
2021 .09
-
Overexpression of Long Non-Coding RNA MIR22HG Represses Proliferation and Enhances Apoptosis via miR-629-5p/TET3 Axis in Osteosarcoma Cells
Journal of Microbiology and Biotechnology
2021 .10
-
P14.5 functions to inhibit cell migration and can be used as a prognostic marker in hepatocellular carcinoma
Genes & Genomics
2023 .01
-
LncRNA H19 Drives Proliferation of Cardiac Fibroblasts and Collagen Production via Suppression of the miR-29a-3p/miR-29b-3p-VEGFA/TGF-β Axis
Molecules and Cells
2022 .03
-
miR-30a Regulates the Expression of CAGE and p53 and Regulates the Response to Anti-Cancer Drugs
Molecules and Cells
2016 .04
-
MiR-200c-3p aggravates gastric cell carcinoma via KLF6
Genes & Genomics
2021 .11
-
miR-101 Inhibiting Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma through Downregulating Girdin
Molecules and Cells
2016 .02
-
Hsa_Circ_0001947/MiR-661/DOK7 Axis Restrains Non-Small Cell Lung Cancer Development
Journal of Microbiology and Biotechnology
2021 .11
-
miRNA-103a-3p Promotes Human Gastric Cancer Cell Proliferation by Targeting and Suppressing ATF7 in vitro
Molecules and Cells
2018 .03
-
The seed sequence is necessary but insufficient for downregulation of target genes by miR-608
Genes & Genomics
2016 .01
-
Suppression of HELLS by miR-451a represses mTOR pathway to hinder aggressiveness of SCLC
Genes & Genomics
2021 .01
-
MicroRNA‑377‑3p inhibits growth and invasion through sponging JAG1 in ovarian cancer
Genes & Genomics
2019 .01
-
Silence of LncRNA GAS5Protects Cardiomyocytes H9c2 against Hypoxic Injury via Sponging miR-142-5p
Molecules and Cells
2019 .01
-
Circulating microRNA expression profiling in young obese Korean women
Nutrition Research and Practice
2020 .08
-
LncSNHG3 promotes hepatocellular carcinoma epithelial mesenchymal transition progression through the miR-152-3p/JAK1 pathway
Genes & Genomics
2022 .01
-
Comparison of the miR-23b and miR-203 Expressions in Endometrial Cancer
대한임상검사과학회지
2017 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0