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논문 기본 정보

자료유형
학술저널
저자정보
Park Bobae (Department of Microbiology 1Department of Microbiology & Immunology Pusan National University S) Yu Sun Nyoung (Department of Microbiology 1Department of Microbiology & Immunology Pusan National University S) Kim Sang-Hun (Section of Pulmonary Critical Care and Sleep Medicine Department of Internal Medicine Yale Universi) Lee Junwon (Department of Biomedicinal Science and Biotechnology Pai Chai University Daejeon 35345 Republic of) Choi Sung Jong (Spine Center Bone Barun Hospital Yangsan 50612 Republic of Korea) Chang Jeong Hyun (Department of Clinical Laboratory Science Daegu Haany University Gyeongsan 38610 Republic of Korea) Yang Eun Ju (Department of Clinical Laboratory Science Daegu Haany University Gyeongsan 38610 Republic of Korea) Kim Kwang-Youn (Korean Medicine Application Center Korea Institute of Oriental Medicine Daegu 41062 Republic of Kor) 안순철 (부산대학교)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제8호
발행연도
2022.8
수록면
1,017 - 1,025 (9page)
DOI
10.4014/jmb.2203.03001

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Bone homeostasis is regulated by constant remodeling through osteogenesis by osteoblasts and osteolysis by osteoclasts and osteoporosis can be provoked when this balance is broken. Present pharmaceutical treatments for osteoporosis have harmful side effects and thus, our goal was to develop therapeutics from intrisincally safe natural products. Fucoidan is a polysaccharide extracted from many species of brown seaweed, with valuable pharmaceutical activities. To intensify the effect of fucoidan on bone homeostasis, we hydrolyzed fucoidan using AMG, Pectinex and Viscozyme. Of these, fucoidan biotransformed by Pectinex (Fu/Pec) powerfully inhibited the induction of tartrate-resistant acid phosphatase (TRAP) activity in osteoclasts differentiated from bone marrow macrophages (BMMs) by the receptor for activation of nuclear factor-κB ligand (RANKL). To investigate potential of lower molecular weight fucoidan it was separated into >300 kDa, 50-300 kDa, and <50 kDa Fu/Pec fractions by ultrafiltration system. The effects of these fractions on TRAP and alkaline phosphatase (ALP) activities were then examined in differentiated osteoclasts and MC3T3-E1 osteoblasts, respectively. Interestingly, 50-300 kDa Fu/Pec suppressed RANKL-induced osteoclasts differentiation from BMMs but did not synergistically enhance osteoblasts differentiation induced by osteogenic agents. In addition, this fraction inhibited the expressions of NFATc1, TRAP, OSCAR, and RANK, which are all key transcriptional factors involved in osteoclast differentiation, and those of Src, c-Fos and Mitf, as determined by RT-PCR. In conclusion, enzymatically low-molecularized 50-300 kDa Fu/Pec suppressed TRAP by downregulating RANKLrelated signaling, contributing to the inhibition of osteoclasts differentiation, and represented a potential means of inducing bone remodeling in the background of osteoporosis.

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