지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제8호
- 발행연도
- 2022.8
- 수록면
- 1,017 - 1,025 (9page)
- DOI
- 10.4014/jmb.2203.03001
이용수
초록· 키워드
오류제보하기
Bone homeostasis is regulated by constant remodeling through osteogenesis by osteoblasts and osteolysis by osteoclasts and osteoporosis can be provoked when this balance is broken. Present pharmaceutical treatments for osteoporosis have harmful side effects and thus, our goal was to develop therapeutics from intrisincally safe natural products. Fucoidan is a polysaccharide extracted from many species of brown seaweed, with valuable pharmaceutical activities. To intensify the effect of fucoidan on bone homeostasis, we hydrolyzed fucoidan using AMG, Pectinex and Viscozyme. Of these, fucoidan biotransformed by Pectinex (Fu/Pec) powerfully inhibited the induction of tartrate-resistant acid phosphatase (TRAP) activity in osteoclasts differentiated from bone marrow macrophages (BMMs) by the receptor for activation of nuclear factor-κB ligand (RANKL). To investigate potential of lower molecular weight fucoidan it was separated into >300 kDa, 50-300 kDa, and <50 kDa Fu/Pec fractions by ultrafiltration system. The effects of these fractions on TRAP and alkaline phosphatase (ALP) activities were then examined in differentiated osteoclasts and MC3T3-E1 osteoblasts, respectively. Interestingly, 50-300 kDa Fu/Pec suppressed RANKL-induced osteoclasts differentiation from BMMs but did not synergistically enhance osteoblasts differentiation induced by osteogenic agents. In addition, this fraction inhibited the expressions of NFATc1, TRAP, OSCAR, and RANK, which are all key transcriptional factors involved in osteoclast differentiation, and those of Src, c-Fos and Mitf, as determined by RT-PCR. In conclusion, enzymatically low-molecularized 50-300 kDa Fu/Pec suppressed TRAP by downregulating RANKLrelated signaling, contributing to the inhibition of osteoclasts differentiation, and represented a potential means of inducing bone remodeling in the background of osteoporosis.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Label-free Noninvasive Characterization of Osteoclast Differentiation Using Raman Spectroscopy Coupled with Multivariate Analysis
Current Optics and Photonics
2017 .08
-
MicroRNA-26a Regulates RANKL-Induced Osteoclast Formation
Molecules and Cells
2015 .01
-
Tectorigenin Promotes Osteoblast Differentiation and in vivo Bone Healing, but Suppresses Osteoclast Differentiation and in vivo Bone Resorption
Molecules and Cells
2018 .03
-
Antimicrobial agents, outer membrane assembly machine, lipoprotein, lipopolysaccharide, outer membrane β-barrel protein, phospholipid
Journal of Microbiology and Biotechnology
2019 .01
-
Rev-erbα Negatively Regulates Osteoclast and Osteoblast Differentiation through p38 MAPK Signaling Pathway
Molecules and Cells
2020 .01
-
Antimetastatic effect of fucoidan against non-small cell lung cancer by suppressing non-receptor tyrosine kinase and extracellular signalrelated kinase pathway
Nutrition Research and Practice
2023 .10
-
Antimetastatic effect of fucoidan against non-small cell lung cancer by suppressing non-receptor tyrosine kinase and extracellular signalrelated kinase pathway
Nutrition Research and Practice
2023 .10
-
갈색거저리 유충 에탄올 추출물이 RANKL에 의해 유도되는 파골세포 분화에 미치는 영향
생명과학회지
2020 .11
-
Enterococcus faecalis induces Inflammatory Bone Diseases by Enhancing Osteoclast Differentiation
한국미생물학회 학술대회논문집
2015 .04
-
Morphological Changes of MG-63 Cells by Fucoidan Treatment
한국미생물학회 학술대회논문집
2016 .04
-
Oleoylethanolamide Exhibits GPR119-Dependent Inhibition of Osteoclast Function and GPR119-Independent Promotion of Osteoclast Apoptosis
Molecules and Cells
2020 .01
-
RANKL 유도된 파골세포 분화에 대한 풀무치 에탄올 추출물의 분화 억제 효과
생명과학회지
2019 .10
-
Fucoidan Attenuates Perfluorooctane Sulfonate-induced Apoptosis of Neuronal Cells
한국해양바이오학회지
2017 .06
-
Augmented Osteoclastogenesis from Committed Osteoclast Precursors by Periodontopathic Bacteria Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis
한국미생물·생명공학회지
2016 .12
-
The Inactivation of ERK1/2, p38 and NF-kB Is Involved in the Down-Regulation of Osteoclastogenesis and Function by A2B Adenosine Receptor Stimulation
Molecules and Cells
2017 .10
-
Current Understanding of RANK Signaling in Osteoclast Differentiation and Maturation
Molecules and Cells
2017 .10
-
Transforming Growth Factor β1/Smad4 Signaling Affects Osteoclast Differentiation via Regulation of miR-155 Expression
Molecules and Cells
2017 .03
-
Calcium-Phosphate Crystals Promote RANKL Expression via the Downregulation of DUSP1
Molecules and Cells
2019 .01
-
Obatoclax Regulates the Proliferation and Fusion of Osteoclast Precursors through the Inhibition of ERK Activation by RANKL
Molecules and Cells
2015 .03
-
PARK2 Induces Osteoclastogenesis through Activation of the NF-κB Pathway
Molecules and Cells
2022 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0