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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.8
- 수록면
- 412 - 420 (9page)
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Multinucleated bone resorptive osteoclasts differentiate from bone marrow-derived monocyte/macrophageprecursor cells. During osteoclast differentiation, mononuclear pre-osteoclasts change their morphology andbiochemical characteristics. In this study, Raman spectroscopy with multivariate techniques such asPrincipal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) were used to extractbiochemical information related to various cellular events during osteoclastogenesis. This technique allowedfor label-free and noninvasive monitoring of differentiating cells, and clearly discriminated four differenttime points during osteoclast differentiation. The Raman band intensity showed significant time-dependentchanges that increased up to day 4. The results of Raman spectroscopy agreed with results from atomicforce microscopy (AFM) and tartrate-resistant acid phosphatase (TRAP) staining, a conventional biologicalassay. Under AFM, normal spindle-like mononuclear pre-osteoclasts became round and smaller at day 2after treatment with a receptor activator of nuclear factor-κB ligand and they formed multinucleated giantcells at day 4. Thus, Raman spectroscopy, in combination with PCA-LDA, may be useful for noninvasivelabel-free quality assessment of cell status during osteoclast differentiation, enabling more efficientoptimization of the bioprocesses.
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참고문헌 (41)
참고문헌 신청
G. A. Rodan / 2000 / Therapeutic approaches to bone diseases / Science 289:1508~1514
W. J. Boyle / 2003 / Osteoclast differentiation and activation / Nature 423:337~342
T. Wada / 2006 / RANKL-RANK signaling in osteoclastogenesis and bone disease / Trends. Mol. Med 12:17~25
G. D. Roodman / 1985 / 1,25-Dihydroxyvitamin D3 causes formation of multinucleated cells with several osteoclast characteristics in cultures of primate marrow / Proc. Natl. Acad. Sci. USA 82:8213~8217
F. Arai / 1999 / Commitment and differentiation of osteoclast precursor cells by the sequential expression of c-Fms and receptor activator of nuclear factor kappaB (RANK) receptors / J. Exp. Med 190:1741~1754
W. J. Boyle / 2003 / Osteoclast differentiation and activation / Nature 423:337~342
T. Wada / 2006 / RANKL-RANK signaling in osteoclastogenesis and bone disease / Trends. Mol. Med 12:17~25
G. D. Roodman / 1985 / 1,25-Dihydroxyvitamin D3 causes formation of multinucleated cells with several osteoclast characteristics in cultures of primate marrow / Proc. Natl. Acad. Sci. USA 82:8213~8217
F. Arai / 1999 / Commitment and differentiation of osteoclast precursor cells by the sequential expression of c-Fms and receptor activator of nuclear factor kappaB (RANK) receptors / J. Exp. Med 190:1741~1754
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