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논문 기본 정보

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학술저널
저자정보
Hideyuki Kinoshita (Chiba University) Sumihisa Orita (Chiba University) Kazuhide Inage (Chiba University) Kazuki Fujimoto (Saiseikai Narashino Hospital) Yasuhiro Shiga (Chiba University) Koki Abe (Chiba University) Masahiro Inoue (Chiba University) Masaki Norimoto (Chiba University) Tomotaka Umimura (Chiba University) Takeshi Ishii (Chiba Cancer Center) Tsukasa Yonemoto (Chiba Cancer Center) Hiroto Kamoda (Chiba Cancer Center) Toshinori Tsukanishi (Chiba Cancer Center) Masahiko Suzuki (Chiba University) Naoya Hirosawa (Chiba University) Tsutomu Akazawa (St. Marianna University School of Medicine) Seiji Ohtori (Chiba University)
저널정보
대한척추외과학회 Asian Spine Journal Asian Spine Journal Vol.14 No.1
발행연도
2020.1
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1 - 8 (8page)

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Study Design: Controlled laboratory study. Purpose: This study aimed to evaluate the in vitro pharmacological activity of growth factors (GFs) in freeze-dried platelet-rich plasma (FD-PRP) after storage for 4 weeks. Overview of Literature: Freshly prepared PRP is a rich source of many GFs. We reported that FD-PRP stored for 8 weeks accelerated bone union in a rat posterolateral fusion model equally well as fresh-PRP. However, the pharmacological activity of FD-PRP after long-term storage has not been shown in vitro. Methods: Immediately after preparation, as well as 4 weeks after freeze-dried storage, the platelet count was measured. Human osteoblasts were treated with fresh-PRP and FD-PRP, respectively. Western blotting was used to assess the phosphorylation of the platelet-derived growth factor (PDGF) receptor (PDGFR) and its downstream target, extracellular signal-regulated kinase (ERK). The proliferation rates of osteoblasts were investigated by immunocytochemistry and MTT cell viability assays. Furthermore, we used western blotting to evaluate the effect of PDGFR knockdown on the phosphorylation of ERK stimulated with fresh-PRP and FD-PRP. Results: Platelet counts in both the fresh-PRP and FD-PRP samples were approximately 10-fold higher than in peripheral blood samples. The phosphorylation and activation of the PDGFR and ERK were evenly induced by fresh-PRP and FD-PRP stimulation. Both fresh-PRP and FD-PRP significantly induced osteoblast proliferation in MTT cell viability assays. Furthermore, osteoblast PDGFR knockdown attenuated the downstream ERK activation by fresh PRP and FD-PRP. Conclusions: We demonstrated the pharmacological activity of PDGF in FD-PRP in vitro after 4 weeks of storage.
#Freeze-dried platelet-rich plasma #Osteoblasts #Proliferation #Platelet-derived growth factor

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참고문헌 (31)

참고문헌 신청
Alsousou J / 2009 / The biology of platelet-rich plasma and its application in trauma and orthopaedic surgery : a review of the literature / J Bone Joint Surg Br 91:987~996 google schola Fallouh L / 2010 / Effects of autologous platelet-rich plasma on cell viability and collagen synthesis in injured human anterior cruciate ligament / J Bone Joint Surg Am 92:2909~2916 google schola Redler LH / 2011 / Platelet-rich plasma therapy : a systematic literature review and evidence for clinical use / Phys Sportsmed 39:42~51 google schola Anitua E / 2004 / Autologous platelets as a source of proteins for healing and tissue regeneration / Thromb Haemost 91:4~15 google schola A Hamid MS / 2014 / Platelet-rich plasma injections for the treatment of hamstring injuries : a randomized controlled trial / Am J Sports Med 42:2410~2418 google schola

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