지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한미용성형외과학회 Archives of Aesthetic Plastic Surgery Archives of Aesthetic Plastic Surgery Vol.21 No.1
- 발행연도
- 2015.1
- 수록면
- 12 - 17 (6page)
이용수
초록· 키워드
오류제보하기
BackgroundPlatelet-rich plasma (PRP) has been advocated as a way to introduce increasedconcentrations of growth factors and other bioactive molecules to injured tissuesin an attempt to optimize the local healing environment. Many methods for PRPpreparation have been introduced. Despite variations in the volume of whole bloodtaken and the efficacy of the platelet concentration, the main objective of PRP preparation is to obtain sufficient platelet concentration in the finally processed autologousplasma. We have been making our own internal primitive PRP preparation, which issafe and aseptic, using simple tubes and a centrifugal separator at the outpatient department base.
MethodsTwenty cc of whole blood was collected and 10 cc of blood was added to eachof two bottles, followed by addition of 1.5 cc adenosine-citrate-dextrose-acid solutionto each bottle. Then, centrifugal separation was performed at 4,000 RPM for 15 minutes. Then, the buffy coat layer was aspirated using a 10 cc syringe equipped with a spinal needle. Platelet activation was initiated by addition of CaCl2 and botropase.
Results We were successful in attaining PRP, which was three folds and six folds concentrated compared with the initial platelet count of whole blood .
ConclusionsOur protocol is economical and only requires a few simple procedures for preparation of PRP. We expect the protocol to be applied to clinical trials without significantcost of time and money.
목차
등록된 정보가 없습니다.
참고문헌 (15)
참고문헌 신청
김승준 / 2007 / 혈소판 농축액이 이식된 지방의 생존에 미치는 영향 / Archives of Plastic Surgery 34(3):291~297
Eppley BL / 2006 / Platelet-rich plasma: a review of biology and applications in plastic surgery / Plast Reconstr Surg 118:147e~159e
Anitua E / 2004 / Autologous platelets as a source of proteins for healing and tissue regeneration / Thromb Haemost 91:4~15
Li H / 2013 / PRP as a new approach to prevent infection: preparation and in vitro antimicrobial properties of PRP / J Vis Exp 74:e50351
Eppley BL / 2004 / Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing / Plast Reconstr Surg 114:1502~1508
Eppley BL / 2006 / Platelet-rich plasma: a review of biology and applications in plastic surgery / Plast Reconstr Surg 118:147e~159e
Anitua E / 2004 / Autologous platelets as a source of proteins for healing and tissue regeneration / Thromb Haemost 91:4~15
Li H / 2013 / PRP as a new approach to prevent infection: preparation and in vitro antimicrobial properties of PRP / J Vis Exp 74:e50351
Eppley BL / 2004 / Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing / Plast Reconstr Surg 114:1502~1508
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