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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 161 - 171 (11page)
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Background Procyanidin B2 (PCB2) can increase the levels of anti-inflammatory and immune mediators. Objectives However, its molecular mechanism in human breast cancer remains unclear. This study aimed to investigate the antitumor effect of PCB2 on MCF-7 cells and to examine the underlying mechanism. Methods The flow cytometry and EdU incorporation assays were measured the PCB2-induced BMECs. The expression levels of inflammatory factors and immune response genes were upregulated in MCF-7 cells, high-throughput sequencing was used to detect differentially expressed genes in blank and PCB2-treated MCF-7 cells. Results The results showed that PCB2 induced the apoptosis of MCF-7 cells. CD36 profiles were affected in MCF-7 cells. Additionally, prediction software identified a miR-145-5p binding site in the CD36 sequence. Luciferase reporter assays and Western blot analysis were used to verify the regulatory relationships between the differentially expressed miRNA miR-145-5p and CD36. MiR-145-5p and its key target (CD36) constitute a potential miRNA-mRNA regulatory pair. Functional studies in MCF-7 cells revealed that CD36 promotes but miR-145-5p inhibits apoptosis. Conclusion Overall, these data suggest that miR-145-5p inhibits the enhancing effect of PCB2 on CD36 expression by binding CD36 and subsequently regulating apoptosis, the immune response and anti-inflammatory pathways. These results provide theoretical and experimental support for the treatment of breast cancer.
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참고문헌 (50)
참고문헌 신청
Adams TS / 2020 / Singlecell RNA-seq reveals ectopic and aberrant lung-resident cell populations in idiopathic pulmonary fbrosis / Sci Adv 6
Alvarez KLF / 2020 / An EdU-based fow cytometry assay to evaluate chicken T lymphocyte proliferation / BMC Vet Res 16:230
Balneaves LG / 2020 / Patient and medical oncologists’ perspectives on prescribed lifestyle intervention-experiences of women with breast cancer and providers / J Clin Med 9
Bi HU / 2020 / Long non-coding RNA H19 promotes osteogenic diferentiation of human bone marrow-derived mesenchymal stem cells by regulating microRNA140–5p/SATB2 axis / J Biosci 45
Boo L / 2020 / Phenotypic and microRNA characterization of the neglected CD24+ cell population in MCF-7 breast cancer 3-dimensional spheroid culture / J Chin Med Ass 83:67~76
Alvarez KLF / 2020 / An EdU-based fow cytometry assay to evaluate chicken T lymphocyte proliferation / BMC Vet Res 16:230
Balneaves LG / 2020 / Patient and medical oncologists’ perspectives on prescribed lifestyle intervention-experiences of women with breast cancer and providers / J Clin Med 9
Bi HU / 2020 / Long non-coding RNA H19 promotes osteogenic diferentiation of human bone marrow-derived mesenchymal stem cells by regulating microRNA140–5p/SATB2 axis / J Biosci 45
Boo L / 2020 / Phenotypic and microRNA characterization of the neglected CD24+ cell population in MCF-7 breast cancer 3-dimensional spheroid culture / J Chin Med Ass 83:67~76
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