지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 888 - 894 (7page)
이용수
초록· 키워드
오류제보하기
It has been reported that silibinin, a natural polyphenolic flavonoid, induces cell death in various cancer cell types. However, the underlying mechanisms by which silibinin induces apoptosis in human glioma cells are poorly understood. The present study was therefore undertaken to examine the effect of silibinin on glioma cell apoptosis and to determine its underlying mechanism in human glioma cells. Apoptosis was estimated by FACS analysis. Reactive oxygen species (ROS) generation and mitochondrial membrane potential (${\Psi}m$) were measured using fluorescence dyes DCFH-DA and $DiOC_6$(3), respectively. Cytochrome c release from mitochondria and caspase-3 activation were estimated by Western blot analysis using specific antibodies. Exposure of cells to 30 mM silibinin induced apoptosis starting at 6 h, with increasing effects after 12-48h in a time-dependent manner. Silibinin caused ROS generation and disruption of ym, which were associated with the silibinin-induced apoptosis. The silibinin-induced ROS generation and disruption in ym were prevented by inhibitors of mitochondrial electron transport chain. The hydrogen peroxide scavenger catalase blocked ROS generation and apoptosis induced by silibinin. Silibinin induced cytochrome c release into cytosolic fraction and its effect was prevented by catalase and cyclosporine A. Silibinin treatment caused caspase-3 activation, which was inhibited by DVED-CHO and cyclosporine A. Pretreatment of caspase inhibitors also protected against the silibinin-induced apoptosis. These findings indicate that ROS generation plays a critical role in the initiation of the silibinin-induced apoptotic cascade by mediation of the mitochondrial apoptotic pathway including the disruption of ${\Psi}m$, cytochrome c release, and caspase-3 activation.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Silibinin Induces Apoptotic Cell Death Via ROS-dependent Mitochondrial Pathway in Human Glioma Cells
동의생리병리학회지
2009 .08
-
大薊의 주성분인 Silibinin이 알레르기 염증반응에 미치는 效果
한방안이비인후피부과학회지
2010 .01
-
Comparative Evaluation of Silibinin Effects on Cell Cycling and Apoptosis in Human Breast Cancer MCF-7 and T47D Cell Lines
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Silibinin Inhibits Proliferation, Induces Apoptosis and Causes Cell Cycle Arrest in Human Gastric Cancer MGC803 Cells Via STAT3 Pathway Inhibition
Asian Pacific journal of cancer prevention : APJCP
2014 .01
-
Protective Effects of Silibinin and Its Possible Mechanism of Action in Mice Exposed to Chronic Unpredictable Mild Stress
Biomolecules & Therapeutics(구 응용약물학회지)
2015 .01
-
Combined treatment of silibinin with either sorafenib or EGFR tyrosine kinase inhibitors enhances growth inhibitory effects in hepatocellular carcinoma cells
Clinical and Molecular Hepatology
2015 .01
-
Silibinin Inhibits LPS-Induced Macrophage Activation by Blocking p38 MAPK in RAW 264.7 Cells
Biomolecules & Therapeutics(구 응용약물학회지)
2013 .01
-
Silibinin attenuates radiation-induced intestinal fibrosis
한국실험동물학회 학술발표대회 논문집
2018 .07
-
Immunosuppressive Effect of Silibinin in Experimental Autoimmune Encephalomyelitis
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
2007 .01
-
Anticancer effect of silibinin on the xenograft model using MDA-MB-468 breast cancer cells
Annals of Surgical Treatment and Research
2014 .09
-
Silibilin-Induces Apoptosis in Breast Cancer Cells by Modulating p53, p21, Bak and Bcl-xl Pathways
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Determination of Silibinin in human plasma by high performance liquid chromatography: validation and application in pharmacokinetic study
대한약학회 학술대회
2001 .01
-
Curcumin and Silibinin Inhibit Telomerase Expression in T47D Human Breast Cancer Cells
Asian Pacific journal of cancer prevention : APJCP
2013 .01
-
TPA-induced growth arrest is increased by silibinin by the down-regulation of cyclin B1 and cdc2
대한외과학회 학술대회 초록집
2009 .11
-
Silibinin attenuates silica dioxide nanoparticles-induced inflammation by suppressing TXNIP/MAPKs/AP-1 signaling
한국실험동물학회 학술발표대회 논문집
2020 .07
-
大薊가 LPS로 유도된 Mouse BV2 Microglial cells의 염증반응에 미치는 영향
대한한방내과학회지
2008 .01
-
Preparation, Characterization and Cytotoxicity of Silibinin-Containing Nanoniosomes in T47D Human Breast Carcinoma Cells
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Silibinin attenuates silica dioxide nanoparticles-induced inflammation by suppressing TXNIP/MAPK/AP-1 signaling
한국실험동물학회 학술발표대회 논문집
2020 .02
-
Silibinin enhances C/EBP$\alpha$ and PPARY expression and induces differentiation of 3T3-L1 preadipocytes
대한약학회 학술대회
2001 .01
-
Arsenic Trioxide Induces Apoptosis in Human Colorectal Adenocarcinoma HT-29 Cells Through ROS
Cancer Research and Treatment
2006 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0