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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한외과학회 Annals of Surgical Treatment and Research Annals of Surgical Treatment and Research Vol.87 No.4
- 발행연도
- 2014.9
- 수록면
- 167 - 173 (7page)
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Purpose: The aim of this study is to know whether silibinin has an anticancer effect on triple negative breast cancer xenograft model using MDA-MB-468 cells.
Methods: To establish the xenograft model, we injected the MDA-MB-468 cells into female Balb/c-nude mice. After establishing a xenograft model, oral silibinin was administered to the tested mice in the way of 200 mg/kg for 45 days. The difference of mean tumor volume between silibinin fed mice and control mice was analyzed. The epidermal growth factor receptor (EGFR) phosphorylation in MDA-MB-468 cells was analyzed by Western blotting. The expression of VEGF, COX-2, and MMP-9 genes in tumor tissue was analyzed by real-time polymerase chain reaction (PCR).
Results: In the xenograft model using MDA-MB-468 cells, we found that oral administration of silibinin significantly suppressed the tumor volume (silibinin treated mice vs. control mice; 230.3 ± 61.6 mm3 vs. 435.7 ± 93.5 mm3, P < 0.001). The phosphorylation of EGFR in MDA-MB-468 cells was inhibited by treatment with 50 mg/mL of silibinin. In real time-PCR analysis of tumor tissue obtained from sacrificed mice, the gene expression of MMP-9, VEGF, and COX-2 was 51.8%?80% smaller in silibinin group than that of control group and we can also verify the similar result using Western blotting analysis.
Conclusion: We verified that silibinin had anticancer effect on xenograft model of MDA-MB-468 cells in the way of preventing the phosphorylation of EGFR and eventually suppressed the production of COX-2, VEGF, and MMP-9 expression. Finally, the tumor volume of xenograft models was decreased after administration of Silibinin.
Methods: To establish the xenograft model, we injected the MDA-MB-468 cells into female Balb/c-nude mice. After establishing a xenograft model, oral silibinin was administered to the tested mice in the way of 200 mg/kg for 45 days. The difference of mean tumor volume between silibinin fed mice and control mice was analyzed. The epidermal growth factor receptor (EGFR) phosphorylation in MDA-MB-468 cells was analyzed by Western blotting. The expression of VEGF, COX-2, and MMP-9 genes in tumor tissue was analyzed by real-time polymerase chain reaction (PCR).
Results: In the xenograft model using MDA-MB-468 cells, we found that oral administration of silibinin significantly suppressed the tumor volume (silibinin treated mice vs. control mice; 230.3 ± 61.6 mm3 vs. 435.7 ± 93.5 mm3, P < 0.001). The phosphorylation of EGFR in MDA-MB-468 cells was inhibited by treatment with 50 mg/mL of silibinin. In real time-PCR analysis of tumor tissue obtained from sacrificed mice, the gene expression of MMP-9, VEGF, and COX-2 was 51.8%?80% smaller in silibinin group than that of control group and we can also verify the similar result using Western blotting analysis.
Conclusion: We verified that silibinin had anticancer effect on xenograft model of MDA-MB-468 cells in the way of preventing the phosphorylation of EGFR and eventually suppressed the production of COX-2, VEGF, and MMP-9 expression. Finally, the tumor volume of xenograft models was decreased after administration of Silibinin.
목차
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (14)
참고문헌 신청
1. [학술지(정기간행물)] - Sorlie T. / 2001 / Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications / Proc Natl Acad Sci U S A 98 : 10869 ~ 10874
2. [학술지(정기간행물)] - Reis-Filho JS. / 2008 / Is acinic cell carcinoma a variant of secretory carcinoma? A FISH study using ETV6 'split apart' probes / Histopathology 52 : 840 ~ 846
3. [학술지(정기간행물)] - Mereish KA. / 1991 / Protection against microcystin-LR-induced hepatotoxicity by Silymarin : biochemistry, histopathology, and lethality / Pharm Res 8 : 273 ~ 277
4. [학술지(정기간행물)] - Singh RP. / 2006 / Effect of silibinin on the growth and progression of primary lung tumors in mice / J Natl Cancer Inst 98 : 846 ~ 855
5. [학술지(정기간행물)] - Jiang C. / 2000 / Anti-angiogenic potential of a cancer chemopreventive flavonoid antioxidant, silymarin : inhibition of key attributes of vascular endothelial cells and angiogenic cytokine secretion by cancer epithelial cells / Biochem Biophys Res Commun 276 : 371 ~ 378
2. [학술지(정기간행물)] - Reis-Filho JS. / 2008 / Is acinic cell carcinoma a variant of secretory carcinoma? A FISH study using ETV6 'split apart' probes / Histopathology 52 : 840 ~ 846
3. [학술지(정기간행물)] - Mereish KA. / 1991 / Protection against microcystin-LR-induced hepatotoxicity by Silymarin : biochemistry, histopathology, and lethality / Pharm Res 8 : 273 ~ 277
4. [학술지(정기간행물)] - Singh RP. / 2006 / Effect of silibinin on the growth and progression of primary lung tumors in mice / J Natl Cancer Inst 98 : 846 ~ 855
5. [학술지(정기간행물)] - Jiang C. / 2000 / Anti-angiogenic potential of a cancer chemopreventive flavonoid antioxidant, silymarin : inhibition of key attributes of vascular endothelial cells and angiogenic cytokine secretion by cancer epithelial cells / Biochem Biophys Res Commun 276 : 371 ~ 378
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UCI(KEPA) : I410-ECN-0101-2015-510-002857705