Preparation, Characterization and Cytotoxicity of Silibinin-Containing Nanoniosomes in T47D Human Breast Carcinoma Cells

Amiri, Boshra; Ebrahimi-Far, Meysam; Saffari, Zahra; Akbarzadeh, Azim; Soleimani, Esmaeil; Chiani, Mohsen

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자료유형: 학술저널

저자정보: Amiri, Boshra (Chemistry faculty, Islamic Azad University of Shahrood) Ebrahimi-Far, Meysam (Department of Toxicology, Faculty of Pharmacy, Islamic Azad University) Saffari, Zahra (Pilot Nanobiotechnology Dep., Pasteur Institute of Iran) Akbarzadeh, Azim (Pilot Nanobiotechnology Dep., Pasteur Institute of Iran) Soleimani, Esmaeil (Chemistry faculty, Islamic Azad University of Shahrood) Chiani, Mohsen (Pilot Nanobiotechnology Dep., Pasteur Institute of Iran)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제17권 제8호

발행연도: 2016.1

수록면: 3,835 - 3,838 (4page)

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Background: Breast cancer is one of the most frequent cancer types within female populations. Silibinin is a chemotherapeutic agent ative against cancer. Niosomes are biodegradable, biocompatible, safe and effective carriers for drug delivery. Objective:To prepare nanoniosomal silibinin and evaluate its cytotoxicity inthe T-47D breast cancer cell line. Materials and Methods: Niosomes were prepared by reverse phase evaporation of a mixture of span 20, silibinin, PEG-2000 and cholesterol in chloroform and methanol solvent (1:2 v/v). The solvent phase was evaporated using a rotary evaporator and the remaining gel phase was hydrated in phosphate buffer saline. Mean size, size distribution and zeta potential of niosomes were measured with a Zetasizer instrument and then nanoparticles underwent scanning electron microscopy. The drug releasing pattern was evaluated by dialysis and the cytotoxicity of nanoniosomes in T-47D cells was assessed by MTT assay. Results: Particle size, size variation and zeta potential of the niosomal nanoparticles were measured as $178.4{\pm}5.4nm$, $0.38{\pm}0.09$ and $-15.3{\pm}1.3mV$, respectively. The amount of encapsulated drug and the level of drug loading were determined $98.6{\pm}2.7%$ and $22.3{\pm}1.8%$, respectively; released drug was estimated about $18.6{\pm}2.5%$ after 37 hours. The cytotoxic effects of nanoniosome were significantly increased when compared with the free drug. Conclusions: This study finding suggests that silibinin nanoniosomes could serve as a new drug formulation for breast cancer therapy.

#Silibinin #nanoniosomes #breast cancer #T-47D cells

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