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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 258 - 263 (6page)
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We demonstrate herein that silibinin, a polyphenolic flavonoid compound isolated from milk thistle (Silybum marianum), inhibits LPS-induced activation of macrophages and production of nitric oxide (NO) in RAW 264.7 cells. Western blot analysis showed silibinin inhibits iNOS gene expression. RT-PCR showed that silibinin inhibits iNOS, TNF-${\alpha}$, and $IL1{\beta}$. We also showed that silibinin strongly inhibits p38 MAPK phosphorylation, whereas the ERK1/2 and JNK pathways are not inhibited. The p38 MAPK inhibitor abrogated the LPS-induced nitrite production, whereas the MEK-1 inhibitor did not affect the nitrite production. A molecular modeling study proposed a binding pose for silibinin targeting the ATP binding site of p38 MAPK (1OUK). Collectively, this series of experiments indicates that silibinin inhibits macrophage activation by blocking p38 MAPK signaling.
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참고문헌 (38)
참고문헌 신청
Al-Anati, L. / 2009 / Silibinin protects OTA-mediated TNF-alpha release from perfused rat livers and isolated rat Kupffer cells / Mol. Nutr. Food Res 53:460~466
Beyaert, R. / 1996 / The p38/RK mitogen-activated protein kinase pathway regulates interleukin-6 synthesis response to tumor necrosis factor / EMBO J 15:1914~1923
Billack, B. / 2006 / Macrophage activation: role of toll-like receptors, nitric oxide, and nuclear factor kappa B / Am. J. Pharm. Educ 70:102
Chen, C. C. / 1999 / p38 but not p44/42 mitogen-activated protein kinase is required for nitric oxide synthase induction mediated by lipopolysaccharide in RAW 264.7 cells / Mol. Pharmacol 55:481~488
Cohen, J. / 2002 / The immunopathogenesis of sepsis / Nature 420:885~891
Beyaert, R. / 1996 / The p38/RK mitogen-activated protein kinase pathway regulates interleukin-6 synthesis response to tumor necrosis factor / EMBO J 15:1914~1923
Billack, B. / 2006 / Macrophage activation: role of toll-like receptors, nitric oxide, and nuclear factor kappa B / Am. J. Pharm. Educ 70:102
Chen, C. C. / 1999 / p38 but not p44/42 mitogen-activated protein kinase is required for nitric oxide synthase induction mediated by lipopolysaccharide in RAW 264.7 cells / Mol. Pharmacol 55:481~488
Cohen, J. / 2002 / The immunopathogenesis of sepsis / Nature 420:885~891
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