Characterization of CEBPA Mutations and Polymorphisms and their Prognostic Relevance in De Novo Acute Myeloid Leukemia Patients

Sarojam, Santhi; Raveendran, Sureshkumar; Vijay, Sangeetha; Sreedharan, Jayadevan; Narayanan, Geetha; Sreedharan, Hariharan

추천

검색

자료유형: 학술저널

저자정보: Sarojam, Santhi (Laboratory of Cytogenetics and Molecular Diagnostics, Division of Cancer Research, Medical College P.O) Raveendran, Sureshkumar (Laboratory of Cytogenetics and Molecular Diagnostics, Division of Cancer Research, Medical College P.O) Vijay, Sangeetha (Laboratory of Cytogenetics and Molecular Diagnostics, Division of Cancer Research, Medical College P.O) Sreedharan, Jayadevan (Research Division, Gulf Medical University) Narayanan, Geetha (Division of Medical Oncology, Regional Cancer Centre, Medical College P.O) Sreedharan, Hariharan (Laboratory of Cytogenetics and Molecular Diagnostics, Division of Cancer Research, Medical College P.O)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제16권 제9호

발행연도: 2015.1

수록면: 3,785 - 3,792 (8page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

The CCAAT/enhancer-binding protein-alpha (CEBPA) is a transcriptional factor that plays a crucial role in the control of proliferation and differentiation of myeloid precursors. This gene was recognized as the target of genetic alterations and were associated with clinical complexity among AML. We here analyze the frequency and types of CEBPA mutations and polymorphisms in a de novo AML patients from South India and tried to find out associations of these variations with different clinical parameters and the prognostic significance in AML. Study was carried out in 248 de novo AML patients, cytogenetic analysis was performed from the bone marrow samples and was karyotyped. PCR-SSCP analysis and sequencing was performed for the detection of CEBPA gene variations. All the statistical analysis was performed using SPSS 17 (statistical package for social sciences) software. Pearson Chi-square test, Mann-Whitney U test, Kaplan-Meier survival analysis and log rank tests were performed. CEBPA mutations were detected in 18% and CEBPA polymorphisms were detected in 18.9% of AML cases studied. Most of the mutations occured at the C terminal region. Polymorphisms were detected in both N and C terminal region. with most common being, c.584_589dup ACCCGC and c.690G>T. A significant association was not observed for the mutation and polymorphism with respect to clinical and laboratory parameters. Survival advantage was observed for the mutated cases compared to non mutated cases, especially for the normal karyotype groups. Polymorphisms has no effect on the survival pattern of AML patients. CEBPA mutation and polymorphisms were observed with similar frequency and was identified in all the FAB subtypes as well as in cytogenetic risk groups in our study population, but CEBPA mutations alone confer a prognostic value for NK AML patients.

#CEBPA #AML #mutation #polymorphism #normal karyotype #cytogenetic risk group

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!