Antileishmanial Activity of Niosomal Combination Forms of Tioxolone along with Benzoxonium Chloride against Leishmania tropica

Maryam Hakimi Parizi; Saeedeh Farajzadeh; Iraj Sharifi; Abbas Pardakhty; Mohammad Hossein Daie Parizi; Hamid Sharifi; Ehsan Salarkia; Saeid Hassanzadeh

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자료유형: 학술저널

저자정보: Maryam Hakimi Parizi (Kerman University of Medical Sciences) Saeedeh Farajzadeh (Kerman University of Medical Sciences) Iraj Sharifi (Kerman University of Medical Sciences) Abbas Pardakhty (Kerman University of Medical Sciences) Mohammad Hossein Daie Parizi (Kerman University of Medical Sciences) Hamid Sharifi (Kerman University of Medical Sciences) Ehsan Salarkia (Kerman University of Medical Sciences) Saeid Hassanzadeh (Shahid Bahonar University of Kerman)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.57 No.4

발행연도: 2019.8

수록면: 359 - 368 (10page)

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In this study, we carried out extensive in vitro studies on various concentrations of tioxolone along with benzoxonium chloride and their niosomal forms against Leishmania tropica. Niosomes were prepared by the hydration method and were evaluated for morphology, size, release study, and encapsulation efficiency. This study measured leishmanicidal activity against promastigote and amastigote, apoptosis and gene expression levels of free solution and niosomal-encapsulated tioxolone along with benzoxonium chloride. Span/Tween 60 niosome had good physical stability and high encapsulation efficiency (more than 97%). The release profile of the entrapped compound showed that a gradual release rate. The combination of niosomal forms on promastigote and amastigote were more effective than glucantime. Also, the niosomal form of this compound was significantly less toxic than glucantime (P≤0.05). The flowcytometric analysis on niosomal form of drugs showed that higher number of early apoptotic event as the principal mode of action (89.13% in 200 μg/ml). Also, the niosomal compound increased the expression level of IL-12 and metacaspase genes and decreased the expression level of the IL-10 gene, which further confirming the immunomodulatory role as the mechanism of action. We observed the synergistic effects of these 2 drugs that induced the apoptotic pathways and also up regulation of an immunomodulatory role against as the main mode of action. Also, niosomal form of this combination was safe and demonstrated strong anti-leishmaniasis effects highlights further therapeutic approaches against anthroponotic cutaneous leishmaniasis in future planning.

#Leishmania tropica #tioxolone #benzoxonium chloride #niosome

참고문헌 (32)

참고문헌 신청

Cortez de Sá J / 2016 / Leishmanicidal, cytotoxicity and wound healing potential of Arrabidaea chica Verlot / BMC Complement Altern Med 16:1

de Vries HJ / 2015 / Cutaneous leishmaniasis : recent developments in diagnosis and management / Am J Clin Dermatol 16:99~109

Plano D / 2011 / Selenocyanates and diselenides : a new class of potent antileishmanial agents / Eur J Med Chem 46:3315~3323

Sharifi F / 2012 / Spatial distribution and molecular identification of Leishmania species from endemic foci of south-eastern Iran / Iran J Parasitol 7:45~52

Kazi KM / 2010 / Niosome : a future of targeted drug delivery systems / J Adv Pharm Technol Res 1:374~380

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