A Novel Anti-Microbial Peptide from Pseudomonas, REDLK Induced Growth Inhibition of Leishmania tarentolae Promastigote In Vitro

Yanhui Yu; Panpan Zhao; Lili Cao; Pengtao Gong; Shuxian Yuan; Xinhua Yao; Yanbing Guo; Hang Dong; Weina Jiang

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자료유형: 학술저널

저자정보: Yanhui Yu (Jilin University) Panpan Zhao (Jilin University) Lili Cao (Jilin University) Pengtao Gong (Jilin University) Shuxian Yuan (Jilin Academy of Animal Husbandry and Veterinary Medicine) Xinhua Yao (Jilin Academy of Animal Husbandry and Veterinary Medicine) Yanbing Guo (Jilin Academy of Animal Husbandry and Veterinary Medicine) Hang Dong (Jilin Academy of Animal Husbandry and Veterinary Medicine) Weina Jiang (Qingdao Municipal Hospital)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.58 No.2

발행연도: 2020.4

수록면: 173 - 179 (7page)

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Leishmaniasis is a prevalent cause of death and animal morbidity in underdeveloped countries of endemic area. However, there is few vaccine and effective drugs. Antimicrobial peptides are involved in the innate immune response in many organisms and are being developed as novel drugs against parasitic infections. In the present study, we synthesized a 5-amino acid peptide REDLK, which mutated the C-terminus of Pseudomonas exotoxin, to identify its effect on the Leishmania tarentolae. Promastigotes were incubated with different concentration of REDLK peptide, and the viability of parasite was assessed using MTT and Trypan blue dye. Morphologic damage of Leishmania was analyzed by light and electron microscopy. Cellular apoptosis was observed using the annexin V-FITC/PI apoptosis detection kit, mitochondrial membrane potential assay kit and flow cytometry. Our results showed that Leishmania tarentolae was susceptible to REDLK in a dose-dependent manner, disrupt the surface membrane integrity and caused parasite apoptosis. In our study, we demonstrated the leishmanicidal activity of an antimicrobial peptide REDLK from Pseudomonas aeruginosa against Leishmania tarentolae in vitro and present a foundation for further research of anti-leishmanial drugs.

#Leishmania tarentolae #Pseudomonas aeruginosa #leishmaniasis #antimicrobial peptide #REDLK

참고문헌 (35)

참고문헌 신청

Desjeux P / 2014 / Leishmaniasis : current situation and new perspectives / Comp Immunol Microbiol Infect Dis 27:305~318

Castillo-Ureta H / 2018 / First report of autochthonous canine leishmaniasis caused by Leishmania (L.) mexicana in Sinaloa, Mexico / Acta Trop 190:253~256

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Griensven J / 2019 / Visceral leishmaniasis : recent advances in diagnostics and treatment regimens / Infect Dis Clin North Am 33:79~99

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