Tamoxifen Induces Apoptosis of Leishmania major Promastigotes in Vitro

Masoud Doroodgar; Mahdi Delavari; Moein Doroodgar; Ali Abbasi; Ali Akbar Taherian; Abbas Doroodgar

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자료유형: 학술저널

저자정보: Masoud Doroodgar (Shahid Beheshti University of Medical Sciences) Mahdi Delavari (Kashan University of Medical Sciences) Moein Doroodgar (Shahid Beheshti University of Medical Sciences) Ali Abbasi (Kashan University of Medical Sciences) Ali Akbar Taherian (Kashan University of Medical Sciences) Abbas Doroodgar (Kashan University of Medical Sciences)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.54 No.1

발행연도: 2016.2

수록면: 9 - 14 (6page)

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Tamoxifen is an antagonist of the estrogen receptor and currently used for the treatment of breast cancer. The current treatment of cutaneous leishmaniasis with pentavalent antimony compounds is not satisfactory. Therefore, in this study, due to its antileishmanial activity, effects of tamoxifen on the growth of promastigotes and amastigotes of Leishmania major Iranian strain were evaluated in vitro. Promastigotes and amastigotes were treated with different concentrations (1, 5, 10, 20, and 50 μg/ml) and time periods (24, 48, and 72 hr) of tamoxifen. After tamoxifen treatment, MTT assay (3-[4,5-dimethylthiazol-2-yl]-2,5 biphenyl tetrazolium bromide assay) was used to determine the percentage of live parasites and Graph Pad Prism software to calculate IC<SUB>50</SUB>. Flow cytometry was applied to investigate the induction of tamoxifen-induced apoptosis in promastigotes. The half maximal inhibitory concentration (IC<SUB>50</SUB>) of tamoxifen on promastigotes was 2.6 μg/ml after 24 hr treatment. Flow cytometry analysis showed that tamoxifen induced early and late apoptosis in Leishmania promastigotes. While after 48 hr in control group the apoptosis was 2.0%, the 50 μg/L concentration of tamoxifen increased it to 59.7%. Based on the in vitro antileishmanial effect, tamoxifen might be used for leishmaniasis treatment; however, further researches on in vivo effects of tamoxifen in animal models are needed.

#Leishmania major #amastigote #promastigote #apoptosis #glucantime #tamoxifen

참고문헌 (21)

참고문헌 신청

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Doroodgar A, Sayyah M, Doroodgar M, Mahbobi S, Nemetian M, Rafizadeh S, Rassi Y. Progressive increasing of cutaneous leishmaniasis in Kashan district, central of Iran. Asian Pac J Trop Dis 2012; 2: 260-263.

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