Genetic Diversity and Phylogenetic Analysis of the Iranian Leishmania Parasites Based on HSP70 Gene PCR-RFLP and Sequence Analysis

Sara Nemati; Asghar Fazaeli; Homa Hajjaran; Ali Khamesipour; Mohsen Falahati Anbaran; Arezoo Bozorgomid; Fatah Zarei

추천

검색

질문

자료유형: 학술저널

저자정보: Sara Nemati (Zanjan University of Medical Sciences) Asghar Fazaeli (Zanjan University of Medical Sciences) Homa Hajjaran (Tehran University of Medical Sciences) Ali Khamesipour (Tehran University of Medical Sciences) Mohsen Falahati Anbaran (University of Tehran) Arezoo Bozorgomid (Tehran University of Medical Sciences) Fatah Zarei (Shahid Beheshti University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.55 No.4

발행연도: 2017.8

수록면: 367 - 374 (8page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Despite the broad distribution of leishmaniasis among Iranians and animals across the country, little is known about the genetic characteristics of the causative agents. Applying both HSP70 PCR-RFLP and sequence analyses, this study aimed to evaluate the genetic diversity and phylogenetic relationships among Leishmania spp. isolated from Iranian endemic foci and available reference strains. A total of 36 Leishmania isolates from almost all districts across the country were genetically analyzed for the HSP70 gene using both PCR-RFLP and sequence analysis. The original HSP70 gene se-quences were aligned along with homologous Leishmania sequences retrieved from NCBI, and subjected to the phylogenetic analysis. Basic parameters of genetic diversity were also estimated. The HSP70 PCR-RFLP presented 3 different electrophoretic patterns, with no further intraspecific variation, corresponding to 3 Leishmania species available in the country, L. tropica, L. major, and L. infantum. Phylogenetic analyses presented 5 major clades, corresponding to 5 species complexes. Iranian lineages, including L. major, L. tropica, and L. infantum, were distributed among 3 complexes L. major, L. tropica, and L. donovani. However, within the L. major and L. donovani species complexes, the HSP70 phylogeny was not able to distinguish clearly between the L. major and L. turanica isolates, and between the L. infantum, L. donovani, and L. chagasi isolates, respectively. Our results indicated that both HSP70 PCR-RFLP and sequence analyses are medically applicable tools for identification of Leishmania species in Iranian patients. However, the reduced genetic diversity of the target gene makes it inevitable that its phylogeny only resolves the major groups, namely, the species complexes.

#HSP70 #Leishmania tropica #Leishmania major #Leishmania infantum #leishmaniasis #phylogenetic analysis #PCR-RFLP #Iran

참고문헌 (36)

참고문헌 신청

[학술지(정기간행물)] - Yaghoobi-Ershadi MR / 2002 / A new focus of cutaneous leishmaniasis caused by Leishmania tropica / Saudi Med J 23 : 291 ~ 294

[학술지(정기간행물)] - Edrisian GhH / 1998 / Visceral leishmaniasis : the Iranian experience / Arch Iran Med 1 : 22 ~ 26

[학술지(정기간행물)] - Mohebali M / 2006 / Application of direct agglutination test(DAT)for the diagnosis and seroepide-miological studies of visceral leishmaniasis in Iran / Iran J Parasitol 1 : 15 ~ 25

[학술지(정기간행물)] - Nadim A / 1978 / Present status of kala-azar in Iran / Am J Trop Med Hyg 27 : 25 ~ 28

[단행본] - Schönian G / 2013 / Drug Resistance in Leishmania Parasites / Springer : 15 ~ 44

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!